کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5847906 | 1561616 | 2015 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Quaternary ammonium salt containing soybean oil: An efficient nanosize gene delivery carrier for halophile green microalgal transformation
ترجمه فارسی عنوان
نمک آمونیوم کواترنری حاوی روغن سویا: یک حامل تحویل ژن کارآمد نانومواد برای تبدیل هیدرولیز سبز
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
چکیده انگلیسی
Dunaliella salina, a halophile green microalga, is considered a robust photobioreactor and a remarkable cost beneficial system for the production of therapeutic recombinant proteins. In this study, with low overall cost, a proper cationic lipid was synthesized from renewable soybean oil as an efficient gene delivery carrier for D. salina cells to create appropriate protein-producing transformed cell lines. To obtain an effective carrier, quaternary ammonium salt containing soybean oil (QASSO) was synthesized through the ring opening reaction of the epoxy groups of epoxidized soybean oil with diethylamine. QASSO was characterized using nuclear magnetic resonance and Fourier-transform infrared instruments. QASSO was used to prepare nanolipoplex construct using plasmid DNA molecules containing green fluorescent protein (GFP) as reporter gene. These nanolipoplexes (QASSO-pGFP, N/PÂ =Â 3) and QASSO had diameter of 63.62 and 110.63Â nm, and zeta potential of â68.89 and 48.25Â mV at pH 7.0, respectively. Results indicated the GFP gene expression and cytoplasmic accumulation of GFP protein in the transformants after incubation under desirable conditions for 48Â h and 1Â week. The transformation efficiency was quantitatively assayed by flow cytometry, which yielded transformations of 58.87% and 48.34% for QASSO and 38.32% and a negligible percentage for Polyfect® after 48Â h and 1Â week incubation, respectively.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemico-Biological Interactions - Volume 225, 5 January 2015, Pages 80-89
Journal: Chemico-Biological Interactions - Volume 225, 5 January 2015, Pages 80-89
نویسندگان
Fariba Akbari, Ahmad Yari Khosroushahi, Hamid Yeganeh,