Dendritic cell migration assay: A potential prediction model for identification of contact allergens

► Methodology for in vitro DC migration assay for identification of contact allergens. ► Assay limitations, critical points and optimization is described. ► Preferential migration of sensitizer exposed MUTZ-LC towards CXCL12 was observed. ► Non-sensitizer-exposed MUTZ-LC only migrated towards CCL5. ► Results were reproducible between three laboratories.