Alteration of delta-6-desaturase (FADS2), secretory phospholipase-A2 (sPLA2) enzymes by Hot-nature diet with co-supplemented hemp seed, evening primrose oils intervention in multiple sclerosis patients

- The co-supplemented oils and Hot-natured diet decreased sPLA2 concentration (sPLA2: secretory Phospholipase A2).
- There is a correlation between MS and a rapid fall of D6D activity (D6D: delta-6-desaturase).
- The co-supplemented oils and Hot-natured diet was associated with decreasing of lipid peroxidation and cell membranes damage in RRMS patients.
- The co-supplemented oils and Hot-natured diet may affect D6D activity.
- Likely, repair of myelin occurred during the early phases of disease by regulation of surface-membrane enzymes.

BackgroundThe effect of nutrition and dietary supplements as environmental factors has been suggested as possible factors affecting both disease risk and progression in on the course of multiple sclerosis with complex genetic-risk profiles. This study was aimed to assess regulation of surface-membrane enzymes such as Delta-6-desaturase (FADS2), secretory Phospholipase A2(sPLA2) by hemp seed and evening primrose oils as well as Hot-natured dietary intervention in relapsing remitting multiple sclerosis (RRMS) patients.Methods and materialsIn this double blind, randomized trial, 100 RRMS patients with Extended disability status score (EDSS)<6 were allocated into 3 groups: “Group A” who received co-supplemented hemp seed and evening primrose oils along with advised Hot nature diet; “Group B”, who received olive oil; “Group C”, who received the co-supplemented oils. Clinically EDSS and functional score as well as biochemical parameters [blood cells polyunsaturated fatty acid (PUFA), FADS2, sPLA2] were assessed at baseline and after 6 months.ResultsMean follow-up was 180 ± 2.9SD days (N = 65, 23 M and 42 F aged 34.25 ± 8.07 years with disease duration 6.80 ± 4.33 years). There was no significant difference in studies parameters at baseline. After 6 months, significant improvements in EDSS and functional score were found in the groups A and C while EDSS and pyramidal score showed significant increase in group B. Alteration of biochemical parameters showed improvement in groups A and C whereas there was worsening condition for group B after the intervention.ConclusionThe co-supplemented hemp seed and evening primrose oils with Hot nature diet can have beneficial effects in improving clinical symptoms and signs in RRMS patients which were confirmed by regulation of surface-membrane enzymes.

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