Differential load-regulated global gene expression in mouse trabecular osteocytes

► We devised a method to extract RNA from trabecular bone osteocytes. ► C5 caudal vertebra was subjected to single and repetitive dose of compression loading. ► Trabecular osteocytic RNA was analyzed for changes in global gene expression. ► We found multiple load-regulated genes. ► Asporin and Wnt5a are genes that may regulate the load-induced bone anabolic effect.