کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5894380 | 1568570 | 2016 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A method for isolating and culturing placental cells from failed early equine pregnancies
ترجمه فارسی عنوان
یک روش برای جداسازی و کشت سلولهای جفت از حاملگی های زودرس
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی تکاملی
چکیده انگلیسی
Early pregnancy loss occurs in 6-10% of equine pregnancies making it the main cause of reproductive wastage. Despite this, reasons for the losses are known in only 16% of cases. Lack of viable conceptus material has inhibited investigations of many potential genetic and pathological causes. We present a method for isolating and culturing placental cells from failed early equine pregnancies. Trophoblast cells from 18/30 (60%) failed equine pregnancies of gestational ages 14-65 days were successfully cultured in three different media, with the greatest growth achieved for cells cultured in AmnioChrome⢠Plus. Genomic DNA of a suitable quality for molecular assays was also isolated from 29/30 of these cases. This method will enable future investigations determining pathologies causing EPL.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Placenta - Volume 38, February 2016, Pages 107-111
Journal: Placenta - Volume 38, February 2016, Pages 107-111
نویسندگان
B.V. Rose, V. Cabrera-Sharp, M.J. Firth, F.E. Barrelet, S. Bate, I.J. Cameron, J.R. Crabtree, J. Crowhurst, A.J. McGladdery, H. Neal, J. Pynn, O.D. Pynn, C. Smith, Z. Wise, K.L.P. Verheyen, D.C. Wathes, A.M. de Mestre,