کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5908652 1570167 2015 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Dual regulatory effects of non-coding GC-rich elements on the expression of virulence genes in malaria parasites
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک بوم شناسی، تکامل، رفتار و سامانه شناسی
پیش نمایش صفحه اول مقاله
Dual regulatory effects of non-coding GC-rich elements on the expression of virulence genes in malaria parasites
چکیده انگلیسی


- We characterized the transcripts of non-coding GC-elements of P. falciparum.
- The transcription of GC-element was required for the activation of a single var gene.
- A transcriptional repression effect of GC-element was linked to heterochromatin.

As the primary virulence factor of falciparum malaria, var genes harboring mutually exclusive expression pattern lead to antigenic variation and immune evasion of this pathogen in human host. Although various mechanisms contribute to silence of var genes, little is known of transcriptional activation pathways of a single var gene and maintenance of its active state with other silent var loci. Here, we report a monoallelic expression pattern of the non-coding GC-elements flanking chromosomal internal var genes, and transcript from the active one was required for activation of the var gene in the same array. Meanwhile, GFP reporter assays revealed a repressive effect on the adjacent gene induced by DNA motifs of the insulator-like GC-element, which was linked to heterochromatin subnuclear localization. Taken together, these data for the first time provide experimental evidence of the dual cis- and trans-acting regulatory functions of the GC-elements in both silence and activation of var genes, which would advance our understanding of the complex regulatory network of the virulence gene family in P. falciparum.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Infection, Genetics and Evolution - Volume 36, December 2015, Pages 490-499
نویسندگان
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