Fluorescence analysis detects gp60 subtype diversity in Cryptosporidium infections

► The hypervariable gp60 gene is used to identify Cryptosporidium subtypes. ► Exposure to the hosts' immune system supports the hypothesis of selection pressure on this gene. ► T-RFLP detected subtype variability within a human infection and mouse passages. ► This study showed that the ratio of subtype populations differs between infections.