کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5950275 | 1172396 | 2010 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Paracrine factors secreted by endothelial progenitor cells prevent oxidative stress-induced apoptosis of mature endothelial cells
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
PEG-SODPBMCMnSODEPCHUVECO2− - O2-ROS - ROSHydrogen peroxide - آب اکسیژنهsuperoxide anion - آنیون سوپر اکسیدOxidative stress - تنش اکسیداتیوApoptosis - خزان یاختهایDihydroethidine - دی هیدروتیدینHuman umbilical vein endothelial cells - سلول های اندوتلیالی ورید ناف انسانperipheral blood mononuclear cells - سلول های تک هسته ای خون محیطیEndothelial progenitor cells - سلول های پیش ساز اندوتلیالEndothelial cells - سلولهای اندوتلیالmanganese superoxide dismutase - سوپر اکسید دیسموتاز منگنزParacrine factors - عوامل پاراکرینCu/ZnSOD - مس / ZnSODcopper/zinc superoxide dismutase - مس / روی سوپراکسید دیسموتازH2O2 - هیدروژن پراکسیدDHE - وReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
کاردیولوژی و پزشکی قلب و عروق
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Paracrine factors secreted by endothelial progenitor cells prevent oxidative stress-induced apoptosis of mature endothelial cells Paracrine factors secreted by endothelial progenitor cells prevent oxidative stress-induced apoptosis of mature endothelial cells](/preview/png/5950275.png)
چکیده انگلیسی
Endothelial progenitor cells (EPC) play a fundamental role in tissue regeneration and vascular repair. Current research suggests that EPC are more resistant to oxidative stress as compared to differentiated endothelial cells. Here we hypothesized that EPC not only possess the ability to protect themselves against oxidative stress but also confer this protection upon differentiated endothelial cells by release of paracrine factors. To test this hypothesis, HUVEC incubated with conditioned medium obtained from early EPC cultures (EPC-CM) were exposed to H2O2 to assess the accumulation of intracellular ROS, extent of apoptosis and endothelial cell functionality. Under oxidative stress conditions HUVEC treated with EPC-CM exhibited substantially lower levels of intracellular oxidative stress (0.2 ± 0.02 vs. 0.4 ± 0.03 relative fluorescence units, p < 0.05) compared to control medium. Moreover, the incubation with EPC-CM elevated the expression level of antioxidant enzymes in HUVEC (catalase: 2.6 ± 0.4; copper/zinc superoxide dismutase (Cu/ZnSOD): 1.6 ± 0.1; manganese superoxide dismutase (MnSOD): 1.4 ± 0.1-fold increase compared to control, all p < 0.05). Furthermore, EPC-CM had the distinct potential to reverse the functional impairment of HUVEC as measured by their capability to form tubular structures in vitro. Finally, incubation of HUVEC with EPC-CM resulted in a significant reduction of apoptosis (0.34 ± 0.01 vs. 1.52 ± 0.12 relative fluorescence units, p < 0.01) accompanied by an increased expression ratio of the anti/pro-apoptotic factors Bcl-2/Bax to 2.9 ± 0.7-fold (compared to control, p < 0.05). Most importantly, neutralization of selected cytokines such as VEGF, HGF, IL-8 and MMP-9 did not significantly reverse the cyto-protective effect of EPC-CM (p > 0.05), suggesting that soluble factors secreted by EPC, possibly via broad synergistic actions, exert strong cyto-protective properties on differentiated endothelium through modulation of intracellular antioxidant defensive mechanisms and pro-survival signals.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Atherosclerosis - Volume 211, Issue 1, July 2010, Pages 103-109
Journal: Atherosclerosis - Volume 211, Issue 1, July 2010, Pages 103-109
نویسندگان
Zijiang Yang, Moritz Wyler von Ballmoos, Daniel Faessler, Jan Voelzmann, Jana Ortmann, Nicolas Diehm, Wiltrud Kalka-Moll, Iris Baumgartner, Stefano Di Santo, Christoph Kalka,