Quantivirus® HPV E6/E7 RNA 3.0 assay (bDNA) is as sensitive, but less specific than Hybrid Capture 2 test

► The percentage of mRNA positive samples increases with the severity of the cytological diagnosis, but not in histological diagnosis. ► E6/E7 mRNA assay had significant higher positivity rate than the Hybrid Capture 2 assay (75.3% versus 62.3%). ► The HPV mRNA assay and the HC2 assay had the same sensitivity of CIN2+. However, the specificity of CIN2+ for the HPV mRNA assay was significantly lower than HC2 assay. ► E6/E7 mRNA assay can be used as auxiliary tool to follow up.