Development of an Aeromonas hydrophila recombinant extracellular protease vaccine

Aeromonas hydrophila (Ah) exists widely in the aquatic environment and infects a variety of animals. Extracellular protease (EPR) is an important protective antigen that induces a specific antibody response to resist Ah infection. In this study, two genes encoding extracellular protease epr2 and epr3 were linked within the expression vector pET32a to construct a recombinant pET-epr2-3 plasmid. The immunogenicity of the fusion protein epr2-3 was investigated as a subunit vaccine in ICR mice. The recombinant epr2-3 protein induced the production of high antibody titers. The survival rate against homogenous Ah J-1 challenge was significantly higher in the epr2-3 vaccinated group (≥80%) compared with the inactivated Ah vaccinated group and the challenge control group (P < 0.01), thus indicating that the recombinant epr2-3 protein provided significant protection against Ah infection. Therefore, the recombinant epr2-3 is a promising candidate for development as a vaccine against Ah infections.

► Extracellular protease genes epr2 and epr3 were linked in an expression vector. ► Recombinant epr2-3 protein expression was analyzed by SDS-PAGE and Western blotting. ► The recombinant epr2-3 protein induced antibody production in ICR mice. ► Recombinant epr2-3 provided Parabramis Pekinensis with significant protection against A. hydrophila infection. ► Recombinant epr2-3 is a promising candidate for subunit vaccine development.