کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6138443 | 1594223 | 2016 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Two asian jumbo phages, ÏRSL2 and ÏRSF1, infect Ralstonia solanacearum and show common features of ÏKZ-related phages
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موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Jumbo phages infecting Ralstonia solanacearum were isolated in Thailand (ÏRSL2) and Japan (ÏRSF1). They were similar regarding virion morphology, genomic arrangement, and host range. Phylogenetic and proteomic tree analyses demonstrate that the ÏRSL2 and ÏRSF1 belong to a group of evolutionary related phages, including Pseudomonas phages ÏKZ, 201Ï2-1 and all previously described ÏKZ-related phages. Despite conserved genomic co-linearity between the ÏRSL2 and ÏRSF1, they differ in protein separation patterns. A major difference was seen in the detection of virion-associated-RNA polymerase subunits. All β- and βâ²-subunits were detected in ÏRSF1, but one βâ²-subunit was undetected in ÏRSL2. Furthermore, ÏRSF1 infected host cells faster (latent period: 60 and 150 min for ÏRSF1 and ÏRSL2, respectively) and more efficiently than ÏRSL2. Therefore, the difference in virion-associated-RNA polymerase may affect infection efficiency. Finally, we show that ÏRSF1 is able to inhibit bacterial wilt progression in tomato plants.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 494, July 2016, Pages 56-66
Journal: Virology - Volume 494, July 2016, Pages 56-66
نویسندگان
Anjana Bhunchoth, Romain Blanc-Mathieu, Tomoko Mihara, Yosuke Nishimura, Ahmed Askora, Namthip Phironrit, Chalida Leksomboon, Orawan Chatchawankanphanich, Takeru Kawasaki, Miyako Nakano, Makoto Fujie, Hiroyuki Ogata, Takashi Yamada,