کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6139491 | 1594238 | 2015 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Heterologous replicase driven 3â² end repair of Cucumber mosaic virus satellite RNA
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ویروس شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
To investigate the extent of the 3â² end repair in a satellite RNA of Cucumber mosaic virus (CMV) strain Q (Qsat) by a heterologous Tomato aspermy virus (TAV), a set of biologically active agrotransformants corresponding to the three genomic RNAs of TAV was developed. Analysis of Nicotiana benthamiana plants agroinfiltrated with TAV and either wild type or each of the six 3â² deletion mutants of Qsat revealed that (i) heterologous replicase failed to generate Qsat multimers, a hallmark feature of homologous replicase dependent replication of Qsat; (ii) manifestation of severe symptom phenotypes and progeny analysis suggested that heterologous replicase was competent to repair Qsat deletion mutants lacking up to 3â²13 nucleotides (nt) but not beyond and (iii) comparative in silico analysis indicated that the 3â² secondary structural features of the repaired Qsat progeny from heterologous vs homologous driven replicases are remarkably very similar. The significance of these observations is discussed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Virology - Volume 478, April 2015, Pages 18-26
Journal: Virology - Volume 478, April 2015, Pages 18-26
نویسندگان
Venkatesh Sivanandam, Erika Varady, A.L.N. Rao,