In vivo electroporation improves therapeutic potency of a DNA vaccine targeting hepadnaviral proteins

► First study on DNA electroporation (EP) in chronic hepadnaviral infection model. ► Importantly, DNA EP enhanced viral clearance form sera and livers of DHBV-carriers. ► DNA EP stimulated a multi-specific B-cell response recognizing neutralizing epitopes. ► DNA EP resulted in high expression of intrahepatic IFN-γ of DHBV-carriers. ► First evidence on benefit of DNA EP for chronic hepatitis B therapy.