کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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6141649 | 1227259 | 2010 | 9 صفحه PDF | دانلود رایگان |
CD8+ T-lymphocytes from HIV-1 infected individuals express unidentified factors that suppress viral replication by inhibiting HIV-1 gene expression. We examined the role of epigenetics in modulating the HIV-1 suppressive factors expressed by primary CD8+ T cells from subjects naturally controlling virus replication. HIV-1 suppression by CD8+ T-lymphocytes was reversed up to 40% by the addition of a histone deacetylase (HDAC) inhibitor. Noncytolytic suppression was not dependent on epigenetic changes within the target cells, as HDAC1 within the target cell was dispensable, and HIV-1 LTR histone acetylation remained unchanged in the presence of CD8+ T-lymphocytes. Histone deacetylation within CD8+ T-lymphocytes was necessary for potent HIV-1 suppression. Blocking HDACs impairs the ability of CD8+ T-lymphocytes to repress HIV-1 transcription, demonstrating that expression of a portion of the suppressive factors is regulated by epigenetics. These data provide a way to focus the search for the suppressive factors and to potentially modulate their expression.
Journal: Virology - Volume 405, Issue 1, 15 September 2010, Pages 234-242