کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6256470 1612940 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Opioid-dependent regulation of high and low fear responses in two inbred mouse strains
ترجمه فارسی عنوان
تنظیم وابسته به اپیوئیتی پاسخ های ترس و تند پایین در دو سویه موسوم به ماوس
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب رفتاری
چکیده انگلیسی


- Low conditioned fear was accompanied by increased expression of Pdyn and Penk.
- High conditioned fear was not associated with altered opioid gene expression.
- Naltrexone and naltrindole restored freezing in stress-resistant mice.
- κ and δ opioid receptor activation alleviated freezing in stress-susceptible mice.
- Selective opioid receptor agonists may protect against trauma-related disorders.

The molecular mechanisms underlying the susceptibility or resilience to trauma-related disorders remain incompletely understood. Opioids modulate emotional learning, but the roles of specific receptors are unclear. Here, we aimed to analyze the contribution of the opioid system to fear responses in two inbred mouse strains exhibiting distinct behavioral phenotypes. SWR/J and C57BL/6J mice were subjected to five consecutive electric footshocks (1 mA each), and the contextual freezing time was measured. Stress-induced alterations in gene expression were analyzed in the amygdala and the hippocampus. In both strains, the fear response was modulated using pharmacological tools. SWR/J mice did not develop conditioned fear but exhibited increased transcriptional expression of Pdyn and Penk in the amygdala region. Blocking opioid receptors prior to the footshocks using naltrexone (2 mg/kg) or naltrindole (5 mg/kg) increased the freezing responses in these animals. The C57BL/6J strain displayed high conditioned fear, although no alteration in the mRNA abundance of genes encoding opioid precursors was observed. Double-injection of morphine (20 mg/kg) following stress and upon context re-exposure prevented the enhancement of freezing. Moreover, selective delta and kappa agonists caused a reduction in conditioned fear responses. To summarize, the increased expression of the Pdyn and Penk genes corresponded to reduced intensity of fear responses. Blockade of the endogenous opioid system restored freezing behavior in stress-resistant animals. The pharmacological stimulation of the kappa and delta opioid receptors in stress-susceptible individuals may alleviate fear. Thus, subtype-selective opioid receptor agonists may protect against the development of trauma-related disorders.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Behavioural Brain Research - Volume 292, 1 October 2015, Pages 95-101
نویسندگان
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