Estradiol directly attenuates sodium currents and depolarizing afterpotentials in isolated gonadotropin-releasing hormone neurons

► In vitro estradiol directly attenuated sodium currents in GnRH neurons from young and aged animals. ► Estradiol modulation of sodium current was time-dependent, and mediated through ERβ. ► Estradiol directly attenuated the DAP; modulation was not time-dependent or mediated through ERβ. ► The DAP was not modulated in neurons from aged animals. ► These results suggest that estradiol regulation of GnRH neuronal output is modulated during aging.