کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6272035 | 1614776 | 2015 | 15 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
An endocannabinoid system is present in the mouse olfactory epithelium but does not modulate olfaction
ترجمه فارسی عنوان
یک سیستم اندو کانابینوئید در اپیتلیوم بویایی موش وجود دارد اما غضروفی را مدوله نمی کند
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کلمات کلیدی
AEAMASH1N-arachidonoylethanolamideDAGLαIP3R3CB22-arachidonylglycerolOMPCB1WIN 55212-2magl2-AGpalmitoylethanolamideoleoylethanolamidePEADAPIGFP2-OG - 2 و4′,6-diamidino-2-phenylindole - 4 '، 6-دیامیدینو-2-فنیلینولOEA - OASolfaction - بویاییprogenitor cells - سلول های پیش گیاهcytokeratin - سیتوکراتینmonoacylglycerol lipase - لیپاز monoacylglycerol لیپازgreen fluorescent protein - پروتئین فلورسنت سبزolfactory marker protein - پروتئین مارکر بویاییWIN - پیروزیCannabinoids - کانابینوئیدcannabinoid type 2 receptor - گیرنده 2 نوع کانابینوئیدcannabinoid type 1 receptor - گیرنده نوع 1 کانابینوئید
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
چکیده انگلیسی
Endocannabinoids modulate a diverse array of functions including progenitor cell proliferation in the central nervous system, and odorant detection and food intake in the mammalian central olfactory system and larval Xenopus laevis peripheral olfactory system. However, the presence and role of endocannabinoids in the peripheral olfactory epithelium have not been examined in mammals. We found the presence of cannabinoid type 1 (CB1) and cannabinoid type 2 (CB2) receptor protein and mRNA in the olfactory epithelium. Using either immunohistochemistry or calcium imaging we localized CB1 receptors on neurons, glia-like sustentacular cells, microvillous cells and progenitor-like basal cells. To examine the role of endocannabinoids, CB1- and CB2- receptor-deficient (CB1â/â/CB2â/â) mice were used. The endocannabinoid 2-arachidonylglycerol (2-AG) was present at high levels in both C57BL/6 wildtype and CB1â/â/CB2â/â mice. 2-AG synthetic and degradative enzymes are expressed in wildtype mice. A small but significant decrease in basal cell and olfactory sensory neuron numbers was observed in CB1â/â/CB2â/â mice compared to wildtype mice. The decrease in olfactory sensory neurons did not translate to impairment in olfactory-mediated behaviors assessed by the buried food test and habituation/dishabituation test. Collectively, these data indicate the presence of an endocannabinoid system in the mouse olfactory epithelium. However, unlike in tadpoles, endocannabinoids do not modulate olfaction. Further investigation on the role of endocannabinoids in progenitor cell function in the olfactory epithelium is warranted.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 300, 6 August 2015, Pages 539-553
Journal: Neuroscience - Volume 300, 6 August 2015, Pages 539-553
نویسندگان
C.R. Hutch, C.J. Hillard, C. Jia, C.C. Hegg,