کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6279659 1615081 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Hypothalamic Npy mRNA is correlated with increased wheel running and decreased body fat in calorie-restricted rats
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
Hypothalamic Npy mRNA is correlated with increased wheel running and decreased body fat in calorie-restricted rats
چکیده انگلیسی


- Caloric restriction (CR) increases wheel running behavior in growing rats.
- We observed a strong positive relationship between wheel running and hypothalamic Npy mRNA in CR but not AL.
- Npy mRNA and body fat were strongly associated in CR but not AL.

The neuro-molecular mechanisms that regulate the relationship between physical activity level, energy homeostasis regulation, and body fat are unclear. Thus, we aimed to investigate the relationship between mRNAs in the hypothalamic arcuate nucleus (ARC) related to energy homeostasis, wheel running distance, and body fat in ad lib (AL) and calorie-restricted (CR) growing rats. We hypothesized that changes in select mRNAs (Pomc, Cart, Agrp, Npy, Lepr, Insr, Mc4r, Ampk, Sirt1, Sirt3) in CR would be associated with decreases in body fat percentage and increased wheel running behavior. Male Wistar rats were given access to voluntary running wheels at 4 weeks of age and randomized into AL (n = 8) and CR (70% of AL; n = 7) groups at 5 weeks of age until study termination at 12 weeks of age. Body composition, serum leptin, insulin, and adiponectin, and ARC mRNA expression in AL and CR rats were assessed and correlated with week-12 running distance to examine potential relationships that may exist. By 12 weeks of age, wheel running was increased ∼3.3-fold (p = 0.03) while body fat percentage was ∼2-fold lower in CR compared to AL (p = 0.001). Compared to AL, ARC Npy mRNA expression was ∼2-fold greater in CR (p = 0.02), while Lepr, Insr, Ampk, and Sirt1 mRNA were additionally increased in CR (p< 0.05). Significant correlations existed between ARC Npy mRNA levels versus week-12 wheel running distance (r = 0.81, p = 0.03), body fat (r = −0.93, p< 0.01), and between body fat and wheel running (r = −0.83, p = 0.02) in CR, but not in AL. These results reveal possible mechanisms by which fat-brain crosstalk may influence physical activity during energy deficit. These data suggest that below a 'threshold' fat content, body fat may drive activity levels, potentially through hypothalamic Npy action.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 618, 8 April 2016, Pages 83-88
نویسندگان
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