کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
6288562 1616254 2015 30 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Reducing activity, glucose metabolism and acid tolerance response of Bacillus cereus grown at various pH and oxydo-reduction potential levels
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
Reducing activity, glucose metabolism and acid tolerance response of Bacillus cereus grown at various pH and oxydo-reduction potential levels
چکیده انگلیسی
Bacillus cereus is a major foodborne bacterial pathogen able to survive a large number of physical-chemical stresses. B. cereus encounters different pH and redox potential (Eh7) levels during its passage through the gastrointestinal tract. Analysis of the combined influence of pH and redox stresses on B. cereus F4430/73 physiology found that B. cereus F4430/73 growth at pH 7.0 at 37 °C had strong reducing capacities, with a total change of 315 mV from an initial redox value of +214 ± 17 mV. The combination of low Eh7 and low pH led to a drastic reduction of growth parameters compared to oxidative Eh7 and neutral pH. Metabolic analysis showed that low pH significantly modifies glucose fermentative metabolism, with changes including decreased production of acid metabolite (acetate, lactate, formate) and increased production of 2,3-butanediol. Low Eh7 slightly enhanced the acid-tolerance response of B. cereus whereas low pH pre-adaptation led to thermal stress cross-protection. These results highlight new mechanisms that bring fresh insight into B. cereus pH and redox stress adaptations.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Microbiology - Volume 46, April 2015, Pages 314-321
نویسندگان
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