Evaluation of a loop-mediated isothermal amplification assay for rapid and simple detection of Vibrio parahaemolyticus in naturally contaminated seafood samples

► We report the LAMP assay for rapid screening of V. parahaemolyticus. ► LAMP showed 100% and 90.8% sensitivity and specificity in 171 seafood samples. ► The conventional culture test requires more than 3 days for identification. ► The LAMP assay requires less than 60 min from the DNA extraction to final detection.