Toxoplasma gondii RH Ankara: Production of evolving tachyzoites using a novel cell culture method

► A novel Toxoplasma gondii cell culture method to facilitate reduction in animal use. ► Production of evolving tachyzoites for the preparation of serological assays'antigen. ► Cost effective for laboratories that has an animal house and cell culture facility. ► Reduction in animal use by cell culture is favorable due to changing ethical values.