کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6352389 | 1622559 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effect of prolonged exposure to sublethal concentrations of DDT and DDE on protein expression in human pancreatic beta cells
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کلمات کلیدی
DTTdichlorodiphenyltrichloroethaneDDEdichlorodiphenyldichloroethylene3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate - 3 - [(3-کلامیدوپروپیل) دی متیل آمونیو] -1-پروپان سولفوناتDMSO - DMSOOCPs - OCP هاOrganochlorine pollutants - آلاینده های آلکیل کلرAlpha-enolase - آلفا انولازDiabetes - بیماری قندDDT - دیکرو دیفنیل تری کلرواتانdithiothreitol - دیتیوتریتولDimethyl sulfoxide - دیمتیل سولفواکسیدcytokeratin - سیتوکراتینCHAPS - چاپس
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Pollution of the environment represents one of less explored potential reasons for the worldwide epidemic of type 2 diabetes. One of the most prevalent organochlorine pollutants remains the pesticide DDT and its degradation product DDE. Despite some epidemiologic correlations between levels of DDT and DDE in human organism and the prevalence of diabetes, there is almost no information about the exact targets of these compounds inside pancreatic beta cells. To detect functional areas of pancreatic beta cells that could be affected by exposure to DDT and DDE, we analyzed changes in protein expression in the NES2Y human pancreatic beta cell line exposed to three sublethal concentrations (0.1 μM, 1 μM, 10 μM) of DDT and DDE for 1 month. Protein separation and identification was achieved using high-resolution 2D-electrophoresis, computer analysis and mass spectrometry. With these techniques, four proteins were found downregulated after exposure to 10 μM DDT: three cytoskeletal proteins (cytokeratin 8, cytokeratin 18 and actin) and one protein involved in glycolysis (alpha-enolase). Two proteins were downregulated after exposure to 10 μM DDE: cytokeratin 18 and heterogenous nuclear ribonucleoprotein H1 (HNRH1). These changes correlate with previously described effects of other stress conditions (e.g. exposure to palmitate, hyperglycemia, imidazoline derivative, and cytokines) on protein expression in pancreatic beta cells. We conclude that cytoskeletal proteins and their processing, glucose metabolism, and mRNA processing may represent targets affected by exposure to conditions hostile to pancreatic beta cells, including exposure to DDT and DDE.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Environmental Research - Volume 142, October 2015, Pages 257-263
Journal: Environmental Research - Volume 142, October 2015, Pages 257-263
نویسندگان
Nela Pavlikova, Pavel Smetana, Petr Halada, Jan Kovar,