Lipotoxicity and steatohepatitis in an overfed mouse model for non-alcoholic fatty liver disease

►The consecutive stages of NAFLD can be recreated in time in an overfed mouse model. ►Pparγ and Fgf21 are induced in the livers of HFLD-overfed mice only. ►Steatohepatitis coincides with obesity, hyperinsulinemia and loss of liver glycogen. ►Steatohepatitis coincides with the occurrence of hepatic endoplasmatic reticulum stress. ►Adipose tissue of overfed mice is inflamed and has reduced adiponectin expression.