Differential mode of attack on membrane phospholipids by an acidic phospholipase A2 (RVVA-PLA2-I) from Daboia russelli venom

► The hydrolysis of membrane phospholipids by RVV acidic PLA2 was studied. ► Catalytic and membrane damaging activities of RVVPLA2‐I are correlated. ► The RVVPLA2‐I preferentially hydrolyzed PC of mitochondrial membrane. ► GC analysis of fatty acids released from different membranes demonstrated distinct results. ► Differential membrane damage suggests the presence of PLA2-sensitive membrane domains.