کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8322854 | 1539886 | 2015 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Oncogenic Human Papillomavirus 16E7 modulates SUMOylation of FoxM1b
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کلمات کلیدی
SUMOshRNAHEK293TCHXPIAS1SUMOylationHPV16 E7human embryonic kidney 293T cellsshort hairpin RNA - RNA موی سر کوتاهDegradation - تنزلCervical cancer - سرطان دهانه رحمcycloheximide - سیکلوهایسیمیدHuman papillomavirus - ویروس پاپیلوم انسانیHPV - ویروس پایپلوم انسانیsmall ubiquitin-like modifier - کوچک مانند ubiquitin مانند اصلاح کننده
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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![عکس صفحه اول مقاله: Oncogenic Human Papillomavirus 16E7 modulates SUMOylation of FoxM1b Oncogenic Human Papillomavirus 16E7 modulates SUMOylation of FoxM1b](/preview/png/8322854.png)
چکیده انگلیسی
The oncogenic transcription factor Forkhead box M1b (FoxM1b), a key regulator of cell cycle, is often overexpressed in many human cancers. Interestingly, posttranslational modifications are known to play important role in regulating the levels and activity of FoxM1b. The purpose of the present study was to characterize the SUMOylation of FoxM1b and identify the functional consequences including viral pathogenesis. Here, we report that FoxM1b interacts with SUMOylating enzymes Ubc9 and PIAS1 and acts as a substrate for SUMOylation. We also show that SUMOylation facilitates FoxM1b protein destabilization and nucleocytoplasmic shuttling. More importantly, we provide the first evidence for a role of E7 oncoprotein in high risk human papillomavirus (HPV) mediated upregulation of FoxM1b. The elevated expression of FoxM1 was determined to be posttranscriptional and was attributed to decreased SUMOylation of FoxM1b in the E7-expressing cells. Moreover, we demonstrate the involvement of SUMOylation in regulation of FoxM1 and present biochemical evidence that HPV16 E7 oncoprotein can modulate SUMOylation of FoxM1b by impairing its interaction with Ubc9. Together, these results provide a novel connection between SUMOylation of FoxM1b and HPV carcinogenesis. The findings may have important implications in the discovery of future anti-cancer therapeutics.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 58, January 2015, Pages 28-36
Journal: The International Journal of Biochemistry & Cell Biology - Volume 58, January 2015, Pages 28-36
نویسندگان
Neha Jaiswal, Rince John, Vaibhav Chand, Alo Nag,