Fabrication and performance characteristics of tough hydrogel scaffolds based on biocompatible polymers

Novel silane crosslinked tough hydrogel scaffolds were prepared using chitosan (CS) and polyvinyl alcohol (PVA) to give network structure and scaffolds properties. The influence of crosslinking and PVA concentration on scaffolds were studied. Fourier transform infrared spectroscopy (FTIR) spectroscopy confirmed the presence of incorporated components. Tensile strength (TS) and fracture strain analysis of scaffolds were detected owing to the mutual effect of chemically and physically crosslinked network. Tough hydrogel scaffolds having 90% CS and 10% PVA exhibited TS of 49.18 MPa and 10.15% elongation at break. The contact angle is less than 90° exhibited the hydrophilic nature of the scaffold. X-ray diffraction analysis (XRD) indicated the characteristics peaks fitting to CS and PVA and increase in the crystallinity of scaffolds. Cytotoxicity of scaffolds with different human fibroblast cell lines (F121, F192 and F84) for indirect method and human dermal fibroblast cell lines (F121) for direct method was evaluated. This indicated that these biomaterials were non-toxic, viable to the used cell lines, helpful in the growth of these cells and did not discharge toxic material damaging to the living cells.