Molecular characterization of a novel trehalose-6-phosphate hydrolase, TreA, from Bacillus licheniformis

► The monomeric trehalose-6-phosphate hydrolase of Bacillus licheniformis remains active in water-miscible organic solvents. ► It experiences an irreversible process toward thermal unfolding and is unfolded by GdnHCl through a reversible pathway. ► These findings provide a platform for the structure-function studies of the enzyme.