کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8356390 | 1542015 | 2018 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Vacuolar processing enzyme (VvβVPE) from Vitis vinifera, processes seed proteins during ovule development, and accelerates seed germination in VvβVPE heterologously over-expressed Arabidopsis
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کلمات کلیدی
VPEVacuolar processing enzymePCDDAFqRT-PCRORFCBBCoomassie Brilliant Blue - Coomassie درخشان آبیExpression - اصطلاحOvule development - توسعه تخمکday after flowering - روز بعد از گلopen reading frame - قاب خواندن بازProgrammed cell death - مرگ برنامهریزی شده یاختهprogrammed cell death (PCD) - مرگ سلولی برنامه ریزی شده (PCD)UTR یا untranslated regions - منطقه ترجمه نشدهuntranslated region - منطقه غیر ترجمهwild type - نوع وحشیVitis vinifera - گیاه انگور، مو
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Vacuolar processing enzymes (VPEs), belonging to cysteine protease, are responsible for processing seed protein during maturation. Stenospermocarpic grapes occur self-abortion in fertilized embryos during the ovule development, which affects the formation of matured seed proteins. However, little is known about VPE functions in ovule self-defeating. Here, we investigated the role of one seed-type VPE gene, VvβVPE. Sequence analysis showed that all ORFs (Open reading frames) of VvβVPE from 19 seed/seedless genotypes are highly conserved. At the transcriptional level, VvβVPE was specifically expressed during ovule development, with distinct expression patterns: it increased gradually in seeded grapes; while weakly expressed in seedless grapes. Whereas, at the translational level, 3 forms of VvβVPE were expressed during ovule development in seeded grape: precursor βVPE (pβVPE), intermediate βVPE (iβVPE) and finally, active mature βVPE (mβVPE). By contrast, in seedless grape, VvβVPE only exists as pβVPE at whole developmental stage of ovule. for confirming these expression patterns, 12 seeded/seedless genotypes were sampled and analyzed. Furthermore, VPE enzyme activity was increased in Arabidopsis overexpressing VvβVPE, leading to faster germination. Our study indicated that VvβVPE is essential for grapevine ovule maturation through various forms and is involved in seed germination.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 274, September 2018, Pages 420-431
Journal: Plant Science - Volume 274, September 2018, Pages 420-431
نویسندگان
Peijie Gong, Yan Li, Yujin Tang, Rong Wei, Zhu Huijun, Yuejin Wang, Chaohong Zhang,