کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8393002 | 1544057 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effect of short- and medium-term toxicity of doxorubicin on spermatogenesis in adult Wistar rats
ترجمه فارسی عنوان
اثر سمیت کوتاه مدت و متوسط مدت دوکسوروبیسین بر اسپرماتوژنز در موش های صحرایی بالغ ویستار
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کلمات کلیدی
موش تست، اسپرماتوژنز، استریولوژی، دوکسوروبیسین،
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
چکیده انگلیسی
Doxorubicin (DXR) is a widely used chemotherapeutic anticancer agent that has potent activity against several solid and non-solid human malignant tumors, including childhood malignancies. However, DXR has serious toxic effects on tissues with rapid cell cycles, such as myeloid and lymphatic tissues, intestinal mucosa, testes and ovaries. In the present study, the short- and medium-term toxic effects of DXR on the reproductive system of male Wistar rats were evaluated using morphometric and stereological tools to quantify damage to the seminiferous epithelium. Adult male Wistar rats were treated with dose of 7.5â¯mg/kg of DXR and were sacrificed at seven, 14, 21 and 28â¯days after treatment. The testes were fixed in glutaraldehyde solution, routinely processed and embedded in plastic for evaluation under a light microscope. A significant reduction in testis weight was found as a result of massive germ cell apoptosis. Differences in comparison to the control group were found in the relative frequency of all stages of the seminiferous epithelium cycle, with significant differences for stages VIII-XI. Apoptosis significantly decreased the number of pachytene spermatocytes in the stages evaluated (I, II-III and VIII) at seven and 14â¯days. At 21 and 28â¯days after treatment, the testes exhibited the massive loss of germ cells that resulted in a missing cell layer. Moreover, reductions in the height of seminiferous tubules, tubular diameter and tubular compartment as well as an increase in the intertubular compartment were found in the period studied.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Reproductive Biology - Volume 18, Issue 2, June 2018, Pages 169-176
Journal: Reproductive Biology - Volume 18, Issue 2, June 2018, Pages 169-176
نویسندگان
Robson Campos Silva, Daniella Maria Coelho Britto, Wagner de Fátima Pereira, Gustavo Eustáquio Alvin Brito-Melo, Cristiane Tolentino Machado, Marcelo Mattos Pedreira,