کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8422715 | 1545960 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A novel flow-system to establish experimental biofilms for modelling chronic wound infection and testing the efficacy of wound dressings
ترجمه فارسی عنوان
سیستم جریان جدید برای ایجاد بیوفیلم های تجربی برای مدل سازی عفونت مزمن زخم و آزمایش اثربخشی سموم زخم
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کلمات کلیدی
بیوفیلم، زخم، استافیلوکوک، سودوموناس، ضد میکروبی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
چکیده انگلیسی
Several models exist for the study of chronic wound infection, but few combine all of the necessary elements to allow high throughput, reproducible biofilm culture with the possibility of applying topical antimicrobial treatments. Furthermore, few take into account the appropriate means of providing nutrients combined with biofilm growth at the air-liquid interface. In this manuscript, a new biofilm flow device for study of wound biofilms is reported. The device is 3D printed, straightforward to operate, and can be used to investigate single and mixed species biofilms, as well as the efficacy of antimicrobial dressings. Single species biofilms of Staphylococcus aureus or Pseudomonas aeruginosa were reproducibly cultured over 72â¯h giving consistent log counts of 8-10 colony forming units (CFU). There was a 3-4 log reduction in recoverable bacteria when antimicrobial dressings were applied to biofilms cultured for 48â¯h, and left in situ for a further 24â¯h. Two-species biofilms of S. aureus and P. aeruginosa inoculated at a 1:1 ratio, were also reproducibly cultured at both 20â¯Â°C and 37â¯Â°C; of particular note was a definitive Gram-negative shift within the population that occurred only at 37â¯Â°C.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbiological Research - Volume 215, October 2018, Pages 141-147
Journal: Microbiological Research - Volume 215, October 2018, Pages 141-147
نویسندگان
Peter F. Duckworth, Richard S. Rowlands, Michele E. Barbour, Sarah E. Maddocks,