کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8426437 | 1546050 | 2018 | 27 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effect of Kisspeptin-10 on plasma luteinizing hormone concentrations and follicular dynamics during the luteal phase in cattle
ترجمه فارسی عنوان
اثر کیسپپتین -10 بر غلظت هورمون لوتئینیزاسیون پلاسما و پویایی فولیکولار در مرحله فاز لوتئال در گاو
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کلمات کلیدی
تلیسه ها، گنادوتروپین ها، تخمدان، کیسپپتین،
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
چکیده انگلیسی
Two experiments were carried out to determine the effect of Kisspeptin-10 on plasma LH concentrations and follicular dynamics during the luteal phase in cattle. We tested the hypothesis that a single treatment of Kisspeptin-10 will increase plasma LH concentration and the diameter of the dominant follicle, and induce ovulation during the luteal phase of the estrous cycle in cattle. In the Experiment 1, Hereford-cross heifers (nâ¯=â¯28, 14-16â¯mo) were given PGF2α im to induce luteolysis and ovulation. On Day 5 (Day 0â¯=â¯ovulation), a new follicular wave was induced by ultrasound-guided follicular ablation. Heifers were treated on Day 10 (4 days after wave emergence) with 100â¯Î¼g GnRH im (nâ¯=â¯9), 2â¯mL saline im (nâ¯=â¯7), 1â¯mg Kisspeptin-10 im (Kp im, nâ¯=â¯6) or 1â¯mg Kisspeptin-10 iv (Kp iv; nâ¯=â¯6). Blood samples were collected at â60, â15, 0, 5, 15min (0â¯minâ¯=â¯time of injection) and every 15â¯min thereafter until 3â¯h. Transrectal ovarian ultrasonography was performed at 12â¯h intervals from Day 10-14. In Experiment 2, non-lactating beef cows on Day 5 were treated with 100â¯Î¼g GnRH im (nâ¯=â¯9), saline im (nâ¯=â¯5), 10â¯mg of Kisspeptin-10 iv (Kp 10â¯mg; nâ¯=â¯5) or 15â¯mg of Kisspeptin-10 iv (Kp 15â¯mg; nâ¯=â¯5). Blood samples were collected at â15, 0, 15, 30, 60, 120, 180â¯min and twice daily ovarian ultrasonography was done from Day 5-10. In Expt 1, plasma LH concentrations increased for 1â¯h following Kp iv administration. The peak concentration occurred at 15â¯min and was higher in the Kp iv group than in the Kp im group (Pâ¯=â¯0.01). The LH peak was 3.5-folds higher in the GnRH group than the Kp iv group (Pâ¯<â¯0.0001). In Expt 2, GnRH induced higher (Pâ¯<â¯0.001) plasma LH concentrations for all time-points than other groups. Kp 15â¯mgâ¯at peak (15min), 30 and 60â¯min induced higher (Pâ¯<â¯0.0001) plasma LH concentrations than Kp 10â¯mg and saline. Kisspeptin-treated animals did not ovulate in either experiment while GnRH induced ovulation (nâ¯=â¯5/9 in Expt 1; 9/9 in Expt 2). The diameter of the dominant follicle was greater (Pâ¯=â¯0.02) at 12-48â¯h after kisspeptin treatment (Kp groups combined) than the Saline group in Expt 2. In conclusion, Kisspeptin-10 increased plasma LH concentrations and follicle size, and although plasma LH concentrations were higher after iv than im administration, but at the doses used, Kisspeptin-10 did not induce ovulation during the luteal phase in cattle.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Theriogenology - Volume 119, 1 October 2018, Pages 268-274
Journal: Theriogenology - Volume 119, 1 October 2018, Pages 268-274
نویسندگان
Carlos E.P. Leonardi, Fernanda C.F. Dias, Gregg P. Adams, Jaswant Singh,