کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8498279 | 1553594 | 2018 | 23 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Stress up-regulates oxidative burst in juvenile Chinook salmon leukocytes
ترجمه فارسی عنوان
استرس تاخیر در مهار اکسیداتیو در نوجوانان چینیوک سلولهای سالمون
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم آبزیان
چکیده انگلیسی
When fish perceive stressful scenarios, their hypothalamus-pituitary-interrenal axis is activated resulting in the release of corticotropin releasing hormone, adrenocorticotropic hormone (ACTH), and finally cortisol. The physiologic stress response of fish has most often been linked to the reduced performance of the immune system, with a few exceptions where the immune system is activated. In this report, we tested the hypothesis that oxidative burst activity levels in juvenile Chinook salmon (Oncorhynchus tshawytscha) are altered when the fish is presented with a stressor. Fish were subjected to a stressor for 3â¯h and then allowed to recover for 20â¯h following the stressor. Plasma and spleens were collected from euthanized fish before the stressor, at the end of a 3â¯h stressor, and 23â¯h after the start of the experiment. Plasma was held at â80â¯Â°C until cortisol radioimmunoassay analysis was performed to confirm stress. Spleens were held in Dulbecco's Modified Eagle Medium overnight and analyzed the day following collection. Oxidative burst activity was measured in splenic leukocytes after being stimulated with phorbol 12-myristate 13-acetate. We found a significant increase in activated oxidative burst from fish subjected to the stressor as compared to unstressed fish. Speculation is given to ACTH being the leukocyte priming agent in this experiment rather than the cortisol itself.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fish & Shellfish Immunology - Volume 80, September 2018, Pages 655-659
Journal: Fish & Shellfish Immunology - Volume 80, September 2018, Pages 655-659
نویسندگان
Crystal L. Herron, K.M. Cogliati, B.P. Dolan, A. Munakata, C.B. Schreck,