Muli bamboo (Melocanna baccifera) leaves ethanolic extract a non-toxic phyto-prophylactic against low pH stress and saprolegniasis in Labeo rohita fingerlings

Bamboo (Melocanna baccifera) plant plays a significant role in traditional Asian medicine and it can be used as an alternative to various chemo-therapeutics or prophylactic agents used in aquaculture. In the present study, the 1st experiment was done for the preparation of extracts from bamboo leaves by using 90% alcohol as a solvent. In the 2nd experiment, toxicity study was carried on Labeo rohita fingerlings (average length of 10.3 ± 1.5 cm and weight 18.5 ± 1.5 g) using BLAL (Bamboo Leaf Alcoholic) extract and it did not show any mortalities in fish even at the dose of 20 g kg−1 body weight, which can be regarded as virtually non-toxic with minimal effect. The 3rd experiment was conducted to find out the effect of BLAL extract based isocaloric and isonitrogenous feed (doses: control- 0.0%, diet T1-0.01%, diet T2-0.1%, diet T3-1% BLAL extract kg−1 feed) on the haemato-immuno-biochemical parameters of L. rohita fingerlings (average length 15.7 ± 1.5 cm and weight 20.2 ± 1.5 g). Regular sampling was done (on 7th, 15th, 30th and 60th day of feeding) for different haematological, immunological and biochemical parameters. The study revealed that the best response occurred within 7 days of feeding with BLAL extract and long-term feeding have shown immunosuppressive condition in the fish. In the 4th experiment, fish were exposed to multiple stressors like low pH stress, and Saprolegnia parastica infection after feeding with BLAL extract (0.1%) based feed for 7 days. The study showed that the BLAL extract can make fish resistant to single stress however, not able to reduce the fish mortality under multiple stresses (S. parastica infection under low pH). Thus, it can be concluded that the BLAL has the potentiality to be used as a non-toxic phyto-prophylactic which can induce non-specific immune response, reduces the low pH stress responses and increases the resistance against saprolegniasis under neutral pH condition in rohu fingerlings.