کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8553965 | 1562697 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effect of Penicillium roqueforti mycotoxins on Caco-2 cells: Acute and chronic exposure
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کلمات کلیدی
Deoxynivalenol(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)MTSMPAFB1OTAFITCDMSO - DMSOOchratoxin A - اکراتوکسین Alethal dose - دوز مرگبارDON - دونDimethylsulfoxide - دیمتیل سولفواکسیدinhibitory concentration - غلظت مهاریfluorescein isothiocyanate - فلوئورسین ایسوتیوسیاناتFumonisin B1 - فومونیزین B1mycophenolic acid - مایکوفنولیک اسید، مایکوفنولاتEuropean Commission - کمیسیون اروپایی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Penicillium roqueforti is a common food and feed contaminant. However, it is also worldwide renowned for its use as a technological culture responsible for the typicity of blue-veined cheese. Members of the P. roqueforti species are also known to be able to produce secondary metabolites including mycophenolic acid (MPA) and roquefortine C (ROQ C) mycotoxins. In order to more closely simulate the reality of mycotoxin exposure through contaminated food consumption, this work investigated the toxicological effects of MPA and ROQ C not only in acute but also in chronic (i.e. 21-days continuous exposure) conditions on Caco-2 cells. Acute exposure to high MPA or ROQ C concentrations induced an increase of IL-8 secretion. Effects of 21-days continuous exposure on barrier integrity, based on concentrations found in blue-veined cheese and mean of blue cheese intake by French consumers, were monitored. Concerning exposure to ROQ C, no alteration of the intestinal barrier was observed. In contrast, the highest tested MPA concentration (780â¯Î¼M) induced a decrease in the barrier function of Caco-2 cell monolayers, but no paracellular passage of bacteria was observed. This study highlighted that exposure to MPA and ROQ C average concentrations found in blue-veined cheese does not seem to induce significant toxicological effects in the tested conditions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 48, April 2018, Pages 188-194
Journal: Toxicology in Vitro - Volume 48, April 2018, Pages 188-194
نویسندگان
Nolwenn Hymery, Jérome Mounier, Emmanuel Coton,