کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8625163 1568112 2018 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Degradation of extracellular matrix regulates osteoblast migration: A microfluidic-based study
ترجمه فارسی عنوان
تخریب ماتریکس خارج سلولی، تنظیم مهاجرت استئوآبلاست: یک مطالعه مبتنی بر میکروپلاستی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی تکاملی
چکیده انگلیسی
Bone regeneration is strongly dependent on the capacity of cells to move in a 3D microenvironment, where a large cascade of signals is activated. To improve the understanding of this complex process and to advance in the knowledge of the role of each specific signal, it is fundamental to analyze the impact of each factor independently. Microfluidic-based cell culture is an appropriate technology to achieve this objective, because it allows recreating realistic 3D local microenvironments by taking into account the extracellular matrix, cells and chemical gradients in an independent or combined scenario. The main aim of this work is to analyze the impact of extracellular matrix properties and growth factor gradients on 3D osteoblast movement, as well as the role of cell matrix degradation. For that, we used collagen-based hydrogels, with and without crosslinkers, under different chemical gradients, and eventually inhibiting metalloproteinases to tweak matrix degradation. We found that osteoblast's 3D migratory patterns were affected by the hydrogel properties and the PDGF-BB gradient, although the strongest regulatory factor was determined by the ability of cells to remodel the matrix.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bone - Volume 107, February 2018, Pages 10-17
نویسندگان
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