کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8645639 | 1569789 | 2018 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular characterization and expression of Piwil1 and Piwil2 during gonadal development and treatment with HCG and LHRH-A2 in Odontobutis potamophila
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کلمات کلیدی
LHRHGTHPGCHPGGnRHORFGSIHCG - اچ سی جیGonad development - توسعه گنادPrimordial germ cell - سلول های بنیادی اولیهgonadosomatic index - شاخص گنادوسوماتیکopen reading frame - قاب خواندن بازluteinizing hormone-releasing hormone - هورمون آزاد کننده هورمون لوتئینیزه کنندهGonadotropin-releasing hormone - هورمون آزاد کننده گنادوتروپینfollicle-stimulating hormone - هورمون تحریک کننده فولیکولluteinizing hormone - هورمون جسم زردFSH - هورمون محرکه فولیکولی Molecular weight - وزن مولکولیGametogenesis - گامتوژنزgonadotropins - گنادوتروپین ها human chorionic gonadotropin - گونادوتروپین کوریونی انسان
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Piwi proteins play an important regulatory role in germ cell division during gametogenesis and gonad development. In order to understand the function of Piwi genes in the reproductive process of the dark sleeper, we identified and characterized Piwil1 and Piwil2 from gonad tissue. The tissue distribution demonstrated that Piwils were highly expressed in the gonad of the dark sleeper. During gonad development, higher expression was observed in stage I of both the testes and ovaries than in subsequent stages at mRNA and protein levels. The results of immunohistochemistry demonstrated that Piwils were predominantly distributed in the spermatogonia, spermatocytes, and early oocytes. When treated with the HPG axis hormone (HCG and LHRH-A2), the expression of Piwils was significantly decreased in the testes and ovaries at mRNA and protein levels. All of these results indicated that Piwils play a vital role in gonad development and gametogenesis. Our findings provide valuable evidence to further clarify the underlying modulation mechanism of Piwils in teleosts.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 647, 20 March 2018, Pages 181-191
Journal: Gene - Volume 647, 20 March 2018, Pages 181-191
نویسندگان
Cheng Zhao, Wenxu Zhu, Shaowu Yin, Quanquan Cao, Hongye Zhang, Xin Wen, Guosong Zhang, Wanli Xie, Shuqiao Chen,