کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8732768 1590545 2017 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Evidência molecular de Borrelia burgdorferi sensu lato em pacientes no centro‐oeste brasileiro
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی طب سالمندان و علم پیری شناسی
پیش نمایش صفحه اول مقاله
Evidência molecular de Borrelia burgdorferi sensu lato em pacientes no centro‐oeste brasileiro
چکیده انگلیسی
We aimed to detect DNA of Borrelia burgdorferi in whole blood and serum samples of patients with clinical symptoms and epidemiology compatible with Brazilian Lyme‐like disease. Four patients with positive epidemiological histories were recruited for the study. Blood samples were collected, screened by serologic testing by ELISA and Western blotting and molecular identification of B. burgdorferi by amplifying a fragment of the conserved gene that synthesizes the hook flagellar (flgE). The results showed positive serology and for the first time, the presence of Borrelia burgdorferi sensu lato in humans in the Midwest region of Brazil. The resulting sequences were similar to GenBank corresponding sequences of Borrelia burgdorferi flgE gene. By neighbor‐joining the phylogenetic analysis, the flgE sequence of the Brazilian strain clustered in a monophyletic group with the sequence of Borrelia burgdorferi sensu lato under 100% bootstrap support. This study opens up promising perspectives and reinforces the need for additional studies to determine the epidemiological characteristics of the disease, as well as the impact of the prevalence of Brazilian borreliosis in Mato Grosso do Sul State, Brazil.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Revista Brasileira de Reumatologia - Volume 57, Issue 6, November–December 2017, Pages 641-645
نویسندگان
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