کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8840843 | 1614699 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Na+, K+-ATPase Activating Antibody Displays in vitro and in vivo Beneficial Effects in the Pilocarpine Model of Epilepsy
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کلمات کلیدی
FRAPAmCaCSFTLE2-NBDGhypometabolismaminomethylcoumarin - آمینو متیل کومارینexcitotoxicity - اکسید سمیتstatus epilepticus - بحران صرعی یا صرع پایدارEpilepsy - بیماری صرعtemporal lobe epilepsy - صرع لوب تمپورالferric-reducing antioxidant power - قدرت آنتی اکسیدانی آهن را کاهش می دهدartificial cerebrospinal fluid - مایع مغزی نخاعی مصنوعیSodium pump - پمپ سدیمPilocarpine - پیلوکارپین
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Na+, K+-ATPase is an important regulator of brain excitability. Accordingly, compelling evidence indicates that impairment of Na+, K+-ATPase activity contributes to seizure activity in epileptic mice and human with epilepsy. In addition, this enzyme is crucial for plasma membrane transport of water, glucose and several chemical mediators, including glutamate, the major excitatory transmitter in the mammalian brain. Since glucose hypometabolism and increased glutamate levels occur in clinical and experimental epilepsy, we aimed the present study to investigate whether activation of Na+, K+-ATPase activity with specific antibody (DRRSAb) would improve glucose uptake and glutamate release in pilocarpine-treated mice. We found decreased uptake of the glucose fluorescent analog 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-il)amino]-2-desoxi-d-glucose (2-NBDG) in cerebral slices from pilocarpine-treated animals. Interestingly, decreased 2-NBDG uptake was not detected in DRRSAb-treated slices, suggesting a protective effect of the Na+, K+-ATPase activator. Moreover, DRRSAb prevented the increase in glutamate levels in the incubation media of slices from pilocarpine-treated mice. In addition, in vivo intrahippocampal injection of DRRSAb restored crossing activity of pilocarpine-treated mice in the open-field test. Overall, the present data further support the hypothesis that activation of the Na+, K+-ATPase is a promising therapeutic strategy for epilepsy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 377, 1 May 2018, Pages 98-104
Journal: Neuroscience - Volume 377, 1 May 2018, Pages 98-104
نویسندگان
Mayara Lütchemeyer de Freitas, Clarissa Vasconcelos de Oliveira, Fernanda Kulinski Mello, VinÃcius Rafael Funck, Michele Rechia Fighera, Luiz Fernando Freire Royes, Ana Flávia Furian, James W. Larrick, Mauro Schneider Oliveira,