کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8949245 | 1645702 | 2018 | 42 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Photoinduction of electron transport on the acceptor side of PSI in Synechocystis PCC 6803 mutant deficient in flavodiiron proteins Flv1 and Flv3
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کلمات کلیدی
DCMUdibromothymoquinoneSynechocystis PCC 6803Photosystems I and IIDBMIBFRLIAC3-(3,4-Dichlorophenyl)-1,1-dimethylurea - 3- (3،4-Dichlorophenyl) -1،1-dimethylureaPSI and PSII - PSI و PSIILET - اجازه دهیدCyclic electron transport - انتقال الکترون سیسیلlinear electron transport - انتقال الکتریکی خطیPhotosynthetic electron transport - حمل و نقل الکترونی فتوسنتزFerredoxin - فریدوکسیینMethyl viologen - متیل ویولونگWhite light - نور سفیدFar-red light - نور قرمز قرمزwild type - نوع وحشیiodoacetamide - یووداکتامید
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
After transferring the dark-acclimated cyanobacteria to light, flavodiiron proteins Flv1/Flv3 serve as a main electron acceptor for PSI within the first seconds because Calvin cycle enzymes are inactive in the dark. Synechocystis PCC 6803 mutant Îflv1/Îflv3 devoid of Flv1 and Flv3 retained the PSI chlorophyll P700 in the reduced state over 10â¯s (Helman et al., 2003; Allahverdiyeva et al., 2013). Study of P700 oxidoreduction transients in dark-acclimated Îflv1/Îflv3 mutant under the action of successive white light pulses separated by dark intervals of various durations indicated that the delayed oxidation of P700 was determined by light activation of electron transport on the acceptor side of PSI. We show that the light-induced redox transients of chlorophyll P700 in dark-acclimated Îflv1/Îflv3 proceed within 2â¯min, as opposed to 1-3â¯s in the wild type, and comprise a series of kinetic stages. The release of rate-limiting steps was eliminated by iodoacetamide, an inhibitor of Calvin cycle enzymes. Conversely, the creation with methyl viologen of a bypass electron flow to O2 accelerated P700 oxidation and made its extent comparable to that in the wild-type cells. The lack of major sinks for linear electron flow in iodoacetamide-treated Îflv1/Îflv3 mutant, in which O2- and CO2-dependent electron flows were impaired, facilitated cyclic electron flow, which was evident from the decreased steady-state oxidation of P700 and from rapid dark reduction of P700 during and after illumination with far-red light. The results show that the photosynthetic induction in wild-type Synechocystis PCC 6803 is largely hidden due to the flavodiiron proteins whose operation circumvents the rate-limiting electron transport steps controlled by Calvin cycle reactions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1859, Issue 10, October 2018, Pages 1086-1095
Journal: Biochimica et Biophysica Acta (BBA) - Bioenergetics - Volume 1859, Issue 10, October 2018, Pages 1086-1095
نویسندگان
Alexander A. Bulychev, Alexander A. Cherkashin, Elena M. Muronets, Irina V. Elanskaya,