کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8950987 | 1645803 | 2018 | 72 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification and characterization of a new satellite-like DNA family in three Agave species
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Repeated DNA analysis was conducted in three Agave species (A. angustifolia Haworth, A. tequilana Weber and A. fourcroydes Lemaire). Self-priming PCR was initially used to generate repeated PCR products and their sequence information was then used to build different primers sets. The ATsat102-113 primer set was able to amplify repeated PCR products, including probably multimers without becoming a typical ladder profile, in all Agave accessions analyzed. Sequence analysis of these amplifications identified two subfamilies of repeated DNA which share six conserved short motifs but exhibit a high genetic distance and different sequence traits between them. Consensus monomers obtained have a length higher than 100â¯bp and are rich in Adenine/Thymine percentage, as many satellites of plants. A representative probe of these consensus monomers was able to hybridize in some PCR amplified products and the more frequent short motif 1 (CTYRAGTTSG) also exhibits probably multimers without becoming a typical ladder profile. These Agave satellite-like subfamilies have different homogenization rates and the subfamily 2 is more conserved. These satellite-like monomers are transcribed and exhibited a strong relation with transposable element signatures of the LTR/Gypsy families and with A. tequilana TSA sequences, some which exhibits transposon-related domains in different locations to the satellite-like sequences identified. FISH analysis of these satellites-like subfamilies only may be reliable determined in the diploid (A. angustifolia Haworth var. Marginata and A. tequilana Weber 'Azul') accessions. In these generally showed a subtelomeric hybridization with less interstitial signals.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Gene - Volume 16, December 2018, Pages 8-18
Journal: Plant Gene - Volume 16, December 2018, Pages 8-18
نویسندگان
Espinosa-Barrera L.A., Sánchez-Teyer L.F., Quiroz-Moreno A., Narváez-Zapata J.A.,