کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8959293 1646302 2018 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The assessment of anti-tumoral activity of polysaccharide extracted from terrestrial filamentous fungus
ترجمه فارسی عنوان
ارزیابی فعالیت ضد تومور پلی ساکارید استخراج شده از قارچ رشته ای زمین
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بوم شناسی
چکیده انگلیسی
Fungal polysaccharides are well-known for the medicinal properties such as antitumor and immunomodulating effects. Hence, this study evaluated antitumor effects of polysaccharide extracted from Fusarium sp. isolated from soil samples of Karaj district, Alborz, Iran along with its taxonomic study. The filamentous fungus strain FK1 was isolated from the soil sample of Karaj, Iran. The strain was identified based on cultural, morphological and 18 S rRNA gene parameters as Fusarium. Further, the strain Fusarium was cultured in fermented broth of modified (PDB) for 10  days at 25 °C. The polysaccharide of strain FK1 was extracted from the mycelium free supernatant by boiling water method and evaluated for antitoxicity effect on two human cancer cell lines: HeLa cell line and Lymphoblastoid cell line (LCL) by MTT method. Findings revealed that water-extracted from mycelia polysaccharide of strain FK1 had the highest cytotoxicity effect against LCL which is the cause of B lymphocyte cancer, at 50  μg/ml concentration dose (114 ± 1.63) followed by 100  μg/ml (105 ± 0.57) and 10  μg/ml (104 ± 0.57), while it did not have a considerable effect on HeLa cell line. Fusarium could be alternative sources as an antitumor component.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Saudi Journal of Biological Sciences - Volume 25, Issue 6, September 2018, Pages 1236-1241
نویسندگان
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