کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9029438 | 1130293 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Enhanced glucose absorption in the rat small intestine following repeated doses of 5-fluorouracil
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کلمات کلیدی
5-FUHEPESD-glucose3-OMGKRbBLMMTXSGLT1DPHCMC-NaBBMV1,6-diphenyl-1,3,5-hexatriene - 1،6-دیفنیل-1،3،5-هگزیدریل3-O-methyl-d-glucose - 3-O-methyl-d-glycoseIntestinal absorption - جذب رودهBrush-border membrane vesicles - حباب های غشایی مرزی برسMembrane fluidity - سیالیت غشاییBasolateral membranes - غشای نازک5-fluorouracil - فلوروراسیل-۵، فلوئورواوراسیلMethotrexate - متوتروکساتcarboxymethyl cellulose sodium salt - کربوکسی متیل سلولز نمک سدیم
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Many studies demonstrated that 5-fluorouracil (5-FU) treatment of rodents caused the damage of small intestine, resulting in the malabsorption, while we recently found that repeated administration of 5-FU to rats increased Na+-dependent glucose absorption in the small intestine. This study investigated the cause of enhanced glucose absorption. 3-O-methyl-d-glucose (3-OMG) absorption was examined using the everted intestine technique. d-Glucose uptake, phlorizin binding, Western blot analysis and membrane fluidity were examined using small intestinal brush-border membrane vesicles (BBMV). Repeated oral administration of 5-FU to rats increased Na+-dependent 3-OMG absorption in the small intestine, while alkaline phosphatase activity in the small intestine decreased. Na+/K+-ATPase activity of 5-FU-treated rats was about three-fold higher than that of control rats. Although the amount of Na+-dependent glucose co-transporter (SGLT1) in 5-FU-treated rats decreased, the overshoot magnitude of d-glucose uptake in BBMV was not altered. Maximum binding of phlorizin in 5-FU-treated rats was 1.5-fold larger than that of control rats, but not altered the maximal rate of d-glucose absorption, Michaelis constant of d-glucose and dissociation constant of phlorizin. The membrane fluidity of 5-FU-treated rats increased. The enhanced d-glucose absorption in 5-FU-treated rats seems to occur secondarily due to the activation of Na+/K+-ATPase activity in basolateral membranes (BLM). Because the amounts of SGLT1 in 5-FU-treated rats decreased, the increase of turnover rate of SGLT1 and/or an expression of unknown Na+-dependent glucose co-transporter with high affinity for d-glucose and phlorizin sensitivity would contribute to the enhancement of d-glucose transport in 5-FU-treated rats.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemico-Biological Interactions - Volume 155, Issue 3, 15 August 2005, Pages 129-139
Journal: Chemico-Biological Interactions - Volume 155, Issue 3, 15 August 2005, Pages 129-139
نویسندگان
Takashi Tomimatsu, Toshiharu Horie,