کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9127168 | 1569975 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Evidence for an evolutionary force that prevents epigenetic silencing between tail-to-tail rice genes with a short spacer
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کلمات کلیدی
InDelRT-PCRRPS20PTGSdsRNAEPSPs3′-UTR - 3'-UTR3′-untranslated region - 3'-ناحیه ترجمه نشده5-enolpyruvylshikimate-3-phosphate synthase - 5-enolpyruvylshikimate-3-فسفات سنتازdouble-stranded RNA - RNA دو رشته ایI/S - استPosttranscriptional gene silencing - خاموش کردن ژن PosttranscriptionalGene silencing - خاموشی ژنinsertion and deletion - درج و حذفCoefficient of Variation - ضریب تغییرWaxy - واکسیناreverse transcription-polymerase chain reaction - واکنش زنجیره ای رونویسی-پلیمراز معکوس
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
During the course of evolution, the genome should have toned down various types of genomic noise, such as those that cause the unstable expression or gene silencing observed in transgenic organisms. We found a rice genomic segment where two genes, encoding 5-enolpyruvylshikimate-3-phosphate synthase (EPSPs) and ribosomal protein small subunit 20 (rps20), are located in a tail-to-tail orientation and separated by only 300 bp of spacer. It is possible that this kind of structure would give rise to unstable expression due to antisense RNA derived from the neighboring gene. We examined this possibility using Northern blot, reverse transcription-polymerase chain reaction (RT-PCR), and 3â² RACE analyses, but obtained no evidence for instability or antisense RNAs of these housekeeping genes. Comparison of the sequences in the corresponding regions among related rice species revealed a lower level of genetic divergence of both the 3â²-untranslated region (3â²-UTRs) than of the other noncoding regions; in particular both of the boundaries between the 3â²-UTRs and the spacer were markedly conserved. The conservation of both the terminal regions is most likely the result of purifying selection, implying a functional role for the strict termination of the transcription of these genes to prevent gene-silencing-related events.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 346, 14 February 2005, Pages 231-240
Journal: Gene - Volume 346, 14 February 2005, Pages 231-240
نویسندگان
So Kobayashi, Yuji Noro, Hironori Nagano, Kaoru T. Yoshida, Toshiyuki Takano-Shimizu, Yuji Kishima, Yoshio Sano,