کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9127356 | 1569976 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effect of 3â²UTR length on the translational regulation of 5â²-terminal oligopyrimidine mRNAs
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کلمات کلیدی
RPS6Ribosomal protein mRNAUTRPABPmRNPmRNAeGFP3′ untranslated region - 3 منطقه غیر ترجمهcDNA - cDNADNA complementary to RNA - DNA مکمل RNAr-protein - r-پروتئینmessenger RNA - RNA messengerTOP - بالاrapid amplification of cDNA ends - تقویت سریع cDNA به پایان می رسدBase pair(s) - جفت پایه (ها)messenger ribonucleoprotein particle - ذرات ریبونولوپروتئین رسولRace - مسابقهuntranslated region - منطقه غیر ترجمهnucleotide(s) - نوکلئوتید (ها)polymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازPoly(A)-binding protein - پروتئین Poly (A) اتصالribosomal protein - پروتئین ریبوزومیenhanced green fluorescent protein - پروتئین فلورسنت سبز افزایش یافته استTranslational control - کنترل انتقال
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In Vertebrates, all genes coding for ribosomal proteins, as well as those for other proteins implicated in the production and function of translation machinery, are regulated by mitogenic and nutritional stimuli, at the translational level. A cis-regulatory element necessary for this regulation is the typical 5â²UTR, common to all ribosomal protein mRNAs, which always starts at the 5â² end with several pyrimidines. Having noticed that the 3â²UTR of all ribosomal protein mRNAs is much shorter than most cellular mRNAs, we have now studied the possible implication of this 3â²UTR feature in the translational regulation. For this purpose, we constructed a number of chimeric genes whose transcribed mRNAs contain: (1) the 5â²UTR of ribosomal protein S6 mRNA or, as a control, of β-actin mRNA; (2) the EGFP reporter coding sequence from the starting AUG to the stop codon; (3) different 3â²UTRs of various lengths. These constructs have been stably transfected in human HEK293 cells, and the translation regulation of the expressed chimeric mRNAs has been analyzed for translation efficiency, in growing and in serum starved cells, by the polysome association assay. The results obtained indicate that, while the typical growth-associated translational regulation is bestowed on an mRNA by the pyrimidine sequence containing 5â²UTR, the stringency of regulation depends on the short size of the 3â²UTR.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 344, 3 January 2005, Pages 213-220
Journal: Gene - Volume 344, 3 January 2005, Pages 213-220
نویسندگان
Mario Ledda, Mara Di Croce, Barbara Bedini, Francesca Wannenes, Marco Corvaro, Pietro Pilo Boyl, Sara Caldarola, Fabrizio Loreni, Francesco Amaldi,