کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9425884 | 1295896 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Behavioral, pharmacological and molecular characterization of the saphenous nerve partial ligation: A new model of neuropathic pain
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کلمات کلیدی
MORPSLSNICCISNLPMSFDRGSPLGAPDHDMSO - DMSOμ Opioid receptor - μ گیرنده اپیوئیدμ opioid receptors - μ گیرنده های اپیوئیدchronic constriction injury - آسیب زدگی مزمنSpared nerve injury - آسیب عصب آسیب دیدهAllodynia - آلودینیا Neuropathic pain - درد نوروپاتیکdimethylsulfoxyde - دی متیل سولفوکسیدSaphenous nerve - عصب صافphenyl methyl sulfonyl fluoride - فنیل متیل سولفونیل فلورایدAnimal models - مدل های حیوانیspinal nerve ligation - پیوند عصب ستون فقراتPartial sciatic nerve ligation - پیوند عصب سیاتیک جزئیdorsal root ganglia - گانگلیس ریشه پشتیglyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژنازcannabinoid receptors - گیرنده های کانابینوئید
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The saphenous partial ligation (SPL) model is a new, easily performed, rodent model of neuropathic pain that consists of a unilateral partial injury to the saphenous nerve. The present study describes behavioral, pharmacological and molecular properties of this model. Starting between 3 and 5 days after surgery, depending on the modality tested, animals developed clear behaviors indicative of neuropathic pain such as cold and mechanical allodynia, and thermal and mechanical hyperalgesia compared with naive and sham animals. These pain behaviors were still present at 1 month. Signs of allodynia also extended to the sciatic nerve territory. No evidence of autotomy or bodyweight loss was observed. Cold and mechanical allodynia but not thermal and mechanical hyperalgesia was reversed by morphine (4 mg/kg i.p.). The cannabinoid receptor agonist WIN 55,212-2 (5 mg/kg i.p.) improved signs of allodynia and hyperalgesia tested except for mechanical hyperalgesia. Gabapentin (50 mg/kg i.p.) was effective against cold and mechanical allodynia but not hyperalgesia. Finally, amitriptyline (10 mg/kg i.p.) failed to reverse allodynia and hyperalgesia and its administration even led to hyperesthesia. Neurobiological studies looking at the expression of μ opioid receptor (MOR), cannabinoid CB1 and CB2 receptors showed a significant increase for all three receptors in ipsilateral paw skin, L3-L4 dorsal root ganglia and spinal cord of neuropathic rats compared with naive and sham animals. These changes in MOR, CB1 and CB2 receptor expression are compatible with what is observed in other neuropathic pain models and may explain the analgesia produced by morphine and WIN 55,212-2 administrations. In conclusion, we have shown that the SPL is an adequate model that will provide a new tool for clarifying peripheral mechanisms of neuropathic pain in an exclusive sensory nerve.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 132, Issue 4, 2005, Pages 1093-1102
Journal: Neuroscience - Volume 132, Issue 4, 2005, Pages 1093-1102
نویسندگان
J.-S. Walczak, V. Pichette, F. Leblond, K. Desbiens, P. Beaulieu,