کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9488801 | 1331384 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effects of IAA and BA on development of globular, heart- and torpedo-stage embryos from cell suspensions of three grape genotypes
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کلمات کلیدی
IAAN6-benzyladenineIBA2,4-D - 2،4-D2,4-dichlorophenoxyacetic acid - 2،4-dichlorophenoxyacetic اسیدindole-3-butyric acid - اسید Indole-3-Butyricindole-3-acetic acid - اسید Indole-3-استیکCell suspension - تعلیق سلولیGrowth regulator - تنظیم کننده رشدSomatic embryo - جنین سمیIn vitro - درونکشتگاهیVitis - ویتیس
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش باغداری
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In order to initiate cell and embryo suspensions, embryogenic calluses derived on NN solid medium with 2,4-D and BA from petioles of in vitro grown plants of three interspecific grapevine hybrids were cultured in three versions of liquid NN medium: (1) without growth regulators, (2) 0.1 mg lâ1 IAA and (3) 0.5 mg lâ1 BA. Cell and embryo suspensions were incubated two and three times in these versions of liquid media in various combinations. Incubating suspensions two times in hormone-free media and/or with 0.1 mg lâ1 IAA led to formation of globular embryos in the three cultivars studied and small numbers of heart-stage embryos in 'Podarok Magaracha' and 'Intervitis Magaracha'. Numerous heart-stage embryos developed in 'Intervitis Magaracha' and 'Podarok Magaracha' when the suspensions had been initiated in medium with 0.5 mg lâ1 BA, and in 'Bianca' this was achieved after two incubations in the above medium. Torpedo-stage embryos were formed after subculturing heart-stage embryo suspensions in medium with 0.1 mg lâ1 IAA in all study cultivars. If only small numbers of embryos of a certain developmental heart- or torpedo- stage were formed, such cell and embryo suspensions need to be repeatedly subcultured in liquid medium with specific growth regulators to enable this process.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Scientia Horticulturae - Volume 104, Issue 3, 15 April 2005, Pages 237-247
Journal: Scientia Horticulturae - Volume 104, Issue 3, 15 April 2005, Pages 237-247
نویسندگان
Valerii A. Zlenko, Ilia V. Kotikov, Leonid P. Troshin,