کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9989742 | 1580764 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
hCMV and Tet promoters for inducible gene expression in rat neurons in vitro and in vivo
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
عصب شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
To advance our understanding of the central nervous system, there is a need for refined approaches to control gene expression in neuronal culture as well as in the brain in vivo. In this study, we have applied a doxycycline-responsive Tet system to obtain a tightly controlled gene expression in neurons. In the absence of doxycycline, the Tet promoter-driven transgene expression was blocked by Tet transrepressor (tTR). Expression was doxycycline activated with the aid of a reverse Tet transactivator (rtTA). Application of both tTR and rtTA resulted in a much greater inducibility, as compared to rtTA alone, mainly due to a decreased basal level of expression. Such effects were observed when tTR and rtTA were driven in cultured neurons by the αCaMKII promoter. However, introduction of the human CMV major immediate-early promoter resulted only in a mediocre neuronal gene expression, unless the cells were treated, either in culture or in vivo, with depolarizing concentrations of KCl. Thus, in the present report, we have examined hCMV and Tet promoter inducibility in neurons to produce an important improvement in the functioning of the Tet system.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neurobiology of Disease - Volume 19, Issues 1â2, JuneâJuly 2005, Pages 283-292
Journal: Neurobiology of Disease - Volume 19, Issues 1â2, JuneâJuly 2005, Pages 283-292
نویسندگان
Witold Konopka, Kamila Duniec, Barbara Mioduszewska, Tomasz Proszynski, Jacek Jaworski, Leszek Kaczmarek,