Keywords: بیان سلولی آزاد; Cell-free expression; Membrane protein; Mitochondrial carrier; Uncoupling protein;
مقالات ISI بیان سلولی آزاد (ترجمه نشده)
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Keywords: بیان سلولی آزاد; Cell-free expression; Membrane protein; Sodium channel;
Keywords: بیان سلولی آزاد; BEST-TROSY; Cell-free expression; Deuteration; Hadamard decoding; Isotope labeling; Membrane proteins; Triple-resonance NMR;
Expression and purification of human phosphatase and actin regulator 1 (PHACTR1) in plant-based systems
Keywords: بیان سلولی آزاد; Cell-free expression; Design of experiments; PHACTR1; Plant cell pack; Plant molecular farming; Transient protein expression;
Folding and stabilizing membrane proteins in amphipol A8-35
Keywords: بیان سلولی آزاد; 3D; three-dimensional; A8-35; a polyacrylate-based amphipol; APol; amphipol; BO; bacterio-opsin; BR; bacteriorhodopsin from Halobacterium salinarum; Ca2+-ATPase; the sarcoplasmic calcium pump; cac; critical aggregation concentration; CD; circular dichrois
The E. coli S30 lysate proteome: A prototype for cell-free protein production
Keywords: بیان سلولی آزاد; GeLC-MS/MS; Pre-fractionation by 1D-SDS-PAGE coupled with liquid chromatography mass spectrometry; FDR; false discovery rate; ABC-transporters; ATP-binding cassette transporters; CECF; continuous exchange cell-free; CFPS; cell-free protein synthesis; em
High-level cell-free expression and functional characterization of a novel aquaporin from Photobactetrium profundum SS9
Keywords: بیان سلولی آزاد; AqpSS9; Cell-free expression; P. profundum SS9; Biomimetic membrane;
The substitution of Proline 168 favors Bax oligomerization and stimulates its interaction with LUVs and mitochondria
Keywords: بیان سلولی آزاد; Bax; Mitochondria; LUV; Cell-free expression; Cell-free assays;
NMR investigation of the isolated second voltage-sensing domain of human Nav1.4 channel
Keywords: بیان سلولی آزاد; CF; cell-free; DC7PC; 1,2-diheptanoyl-sn-glycero-3-phosphocholine; DDM; n-dodecyl β-d-maltoside; DHPC; 1,2-dihexanoyl-sn-glycero-3-phosphocholine; DMPC; 1,2-dimiristoyl-sn-glycero-3-phosphocholine; DMPG; 1,2-dimiristoyl-sn-glycero-3-phosphoglycerol; DOPG
Chaperonin-enhanced Escherichia coli cell-free expression of functional CXCR4
Keywords: بیان سلولی آزاد; Chaperonin; G protein-coupled receptor; Cell-free expression; Protein folding; Structural stability; Biological activity
Soluble full-length expression and characterization of snRNP protein U1-68/70 K
Keywords: بیان سلولی آزاد; Cell-free expression; Autoimmunity; snRNP complex; Chaperones
LC-MS/MS characterization of combined glycogenin-1 and glycogenin-2 enzymatic activities reveals their self-glucosylation preferences
Keywords: بیان سلولی آزاد; CID; collision-induced dissociation; DTT; dithiothreitol; ECD; electron capture dissociation; Glc; glucose; HEPES; 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; HEK; Human Embryonic Kidney; LC; liquid chromatography; MS; mass spectrometry; PAS; peri
Functional properties of cell-free expressed human endothelin A and endothelin B receptors in artificial membrane environments
Keywords: بیان سلولی آزاد; Aso-PC; soybean l-α-phosphatidylcholine; BTE; brain total extract; CECF; continuous exchange cell-free; CF; cell-free; CHS; cholesteryl hemisuccinate; CVs; column volumes; DMPC; 1,2-dimyristoyl-sn-glycero-3-phosphocholine; DMPG; 1,2-dimyristoyl-sn-glycer
A second rhodopsin-like protein in Cyanophora paradoxa: Gene sequence and protein expression in a cell-free system
Keywords: بیان سلولی آزاد; Rhodopsin-like protein; Cyanophora paradoxa; CpR2; Cell-free expression; Absorption spectrum
Cell-free expression of human glucosamine 6-phosphate N-acetyltransferase (HsGNA1) for inhibitor screening
Keywords: بیان سلولی آزاد; GNA1; Cell-free expression; Inhibitor screening; Simulated annealing;
Pv12, a 6-Cys antigen of Plasmodium vivax, is localized to the merozoite rhoptry
Keywords: بیان سلولی آزاد; Plasmodium vivax; Merozoite; Pv12; Rhoptry; Cell-free expression; 6-Cys domain; Humoral immune response
Molecular pathogenesis of a new glycogenosis caused by a glycogenin-1 mutation
Keywords: بیان سلولی آزاد; Glycogen; Glycogenin; Glycogen storage disease; Cell-free expression; Mass spectrometry
Expression screening of membrane proteins with cell-free protein synthesis
Keywords: بیان سلولی آزاد; Membrane protein; Cell-free expression; Circular dichroism; Batch;
Characterization of co-translationally formed nanodisc complexes with small multidrug transporters, proteorhodopsin and with the E. coli MraY translocase
Keywords: بیان سلولی آزاد; AFM; atomic force microscopy; BR; bacteriorhodopsin; Cd; cardiolipin 1â²,3â²-bis[1,2-dimyristoyl-sn-glycero-3-phospho]-sn-glycerol; CECF; continuous exchange cell-free; CF; cell-free; DDM; n-dodecyl-β-d-maltopyranoside; DHPC; 1,2-diheptanoyl-sn-glycero
A systematic approach to increase the efficiency of membrane protein production in cell-free expression systems
Keywords: بیان سلولی آزاد; Cell-free expression; Tag variation; Initiation of translation; GPCRs; P-CF; GPCRs; Thermostability;
Lipid-protein nanodiscs for cell-free production of integral membrane proteins in a soluble and folded state: Comparison with detergent micelles, bicelles and liposomes
Keywords: بیان سلولی آزاد; Brij-35; dodecylpolyoxyethylene 35; Brij-58; hexadecylpolyoxyethylene 58; Brij-78; octadecylpolyoxyethylene 78; Brij-98; octadecylpolyoxyethylene 98; CF; cell-free; DC7PC; 1,2-diheptanoyl-sn-glycero-3-phosphocholine; DHPC; 1,2-dihexanoyl-sn-glycero-3-phos
Plasmodial ortholog of Toxoplasma gondii rhoptry neck protein 3 is localized to the rhoptry body
Keywords: بیان سلولی آزاد; Cell-free expression; Erythrocyte invasion; Merozoite; Plasmodium falciparum; Rhoptry
α-Hemolysin pore formation into a supported phospholipid bilayer using cell-free expression
Keywords: بیان سلولی آزاد; QCM-D; quartz crystal microbalance-dissipation; SPB; supported phospholipid bilayer; AFM; atomic force microscopy; eGFP; enhanced Green Fluorescent Protein; αHL-eGFP; α-hemolysin-eGFP fusion protein; PBS; phosphate buffered saline; EggPC; l-α-lysophosp
Crystallization of the membrane protein hVDAC1 produced in cell-free system
Keywords: بیان سلولی آزاد; Membrane protein; Voltage-dependent anion channel; Cell-free expression; Crystallization
Expression of neurotransmitter transporters for structural and biochemical studies
Keywords: بیان سلولی آزاد; VMAT; Membrane proteins; Cell-free expression; Baculovirus; Insect cells
Modulation of G-protein coupled receptor sample quality by modified cell-free expression protocols: A case study of the human endothelin A receptor
Keywords: بیان سلولی آزاد; B35; Brij-35; B58; Brij-58; B78; Brij-78; B98; Brij-98; CD; circular dichroism; CF; cell-free; CHAPS; 3-[(3-cholamidopropyl) dimethylammonio]-1-propansulphonat; CHS; cholesteryl hemisuccinate tris salt; CMC; critical micellar concentration; CV; column vol
Pyruvate kinase type-II isozyme in Plasmodium falciparum localizes to the apicoplast
Keywords: بیان سلولی آزاد; Plasmodium falciparum; Pyruvate kinase II; Apicoplast; Mitochondria; Cell-free expression
Biogenesis of cytochrome c oxidase - in vitro approaches to study cofactor insertion into a bacterial subunit I
Keywords: بیان سلولی آزاد; BCA; 2,2â²-bicinchoninic acid; CMC; critical micelle concentration; COX; cytochrome c oxidase; EXAFS; extended X-ray absorption fine structure; IMM; inner mitochondrial membrane; IMS; intermembrane space; SU; subunit; TEV; tobacco etch virus; TXRF; total
Preparative scale cell-free expression systems: New tools for the large scale preparation of integral membrane proteins for functional and structural studies
Keywords: بیان سلولی آزاد; Integral membrane proteins; Structural analysis; Solubilization; Reconstitution; Detergent; NMR spectroscopy; G-protein coupled receptors; β-barrel proteins; Multidrug resistance; Cell-free expression; Detergent micelles; Transporter; Liposomes; Labelling
Experimental and Computational Analysis of Translation Products in Apomyoglobin Expression
Keywords: بیان سلولی آزاد; cell-free expression; E. coli; incomplete translation; elongation; kineticsapoMb, sperm whale apomyoglobin; CDTA, trans-1,2-diaminocyclohexane-N,N,N′,N′-tetraacetic acid; IC, incomplete chain; Chaps, 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfona
Ribosome display of antibodies: expression, specificity and recovery in a eukaryotic system
Keywords: بیان سلولی آزاد; ARM; antibody-ribosome-mRNA complex; PBS; phosphate buffered saline; Prog-11α-HMS; progesterone-11α-hemisuccinate; Prog-11α-HMS-BSA; progesterone-11α-hemisuccinyl-BSA; Prog-3-CMO; progesterone-3-carboxymethyloxime; Prog-6β-HMS; progesterone-6β-hemis