Keywords: FPLC، کروماتوگرافی مایع سریع پروتئین; CC, cardiac cachexia; CHF, chronic heart failure; CV, coefficient of variation; DXA, dual energy X-ray absorptiometry; eGFR, estimated glomerular filtration rate; FM, fat mass; FFM, fat free mass; FPLC, fast protein liquid chromatography; Hb1Ac, glycosyla
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Non-disulfide-bridged peptides from Tityus serrulatus venom: Evidence for proline-free ACE-inhibitors
Keywords: FPLC، کروماتوگرافی مایع سریع پروتئین; ACE, angiotensin converting enzyme; BgNav, Blattella germanica sodium channel; BK, bradykinin; BPPs, bradykinin-potentiating peptides; CPE, cytopathic effect; DBPs, disulfide-bridged peptides; FPLC, Fast Protein Liquid Chromatography; Kv, voltage gated po
Astaxanthin reduces hepatic lipid accumulations in high-fat-fed C57BL/6J mice via activation of peroxisome proliferator-activated receptor (PPAR) alpha and inhibition of PPAR gamma and Akt
Keywords: FPLC، کروماتوگرافی مایع سریع پروتئین; ACC1, acetyl-CoA carboxylase 1; ACOX1, acyl-CoA oxidase 1; Akt, protein kinase B; AX, astaxanthin; CPT1, carnitine palmitoyltransferase 1; FAS, fatty acid synthase; FPLC, fast protein liquid chromatography; GSK-3, glycogen synthase kinase-3; HFD, high-fat
Optimization of expression and purification of HSPA6 protein from Camelus dromedarius in E. coli
Keywords: FPLC، کروماتوگرافی مایع سریع پروتئین; amp, ampicillin; DTT, dithiothreitol; EDTA, ethylenediaminetetraacetic acid; FPLC, fast protein liquid chromatography; IPTG, isopropyl β-d-1-thiogalactopyranoside; LB, Luria–Bertani; 2× LB, double strength Luria–Bertani; NB, nutrient broth; Ni–NTA, nickel
Structural and biochemical characterization of two heme binding sites on α1-microglobulin using site directed mutagenesis and molecular simulation
Keywords: FPLC، کروماتوگرافی مایع سریع پروتئین; A1M, α1-microglobulin; AMBP, A1M-bikunin precursor protein; IgA, immunoglobulin A; ROS, reactive oxygen species; FPLC, fast protein liquid chromatography; SPR, surface plasmon resonance; MD, molecular dynamics; CD, circular dichroism; RMSD, root mean squa
Prokaryotic overexpression of TEV–rhGH and characterization of its polyclonal antibody
Keywords: FPLC، کروماتوگرافی مایع سریع پروتئین; BSA, bovine serum albumin; DTT, dithiothreitol; ELISA, enzyme-linked immunosorbent assay; FPLC, fast protein liquid chromatography; HRP, horseradish peroxidase; IPTG, isopropyl β-D-thiogalactoside; NHS, N-hydroxysuccinimide ester; Ni-NTA, nickel-charged n
Isolation and characterization of peptides with dipeptidyl peptidase-IV inhibitory activity from pepsin-treated bovine whey proteins
Keywords: FPLC، کروماتوگرافی مایع سریع پروتئین; α-la, α-lactalbumin; DPP-IV, dipeptidyl peptidase-IV; FPLC, fast protein liquid chromatography; GIP, glucose-dependent insulinotropic polypeptide; GLP-1, glucagon-like peptide-1; Gly-Pro-p-NA, Gly-Pro-para-nitroanilide; IC50, concentration causing 50% inh
Generation and characterization of nanobodies against rhGH expressed as sfGFP fusion protein
Keywords: FPLC، کروماتوگرافی مایع سریع پروتئین; AP, alkaline phosphatase; BCIP, 5-bromo-4-chloro-3-indolyl phosphate; BSA, bovine serum albumin; ELISA, enzyme-linked immunosorbent assay; FPLC, fast protein liquid chromatography; GHBP, growth hormone binding protein; HCAb, heavy-chain antibody; HRP, hor
Chain conformation and immunomodulatory activity of a hyperbranched polysaccharide from Cordyceps sinensis
Keywords: FPLC، کروماتوگرافی مایع سریع پروتئین; ELSD, evaporative light-scattering detector; FPLC, fast protein liquid chromatography; FT-IR, Fourier transform-infrared spectroscopy; GC–MS, gas chromatography–mass spectrometry; HPSEC, high performance size exclusion chromatography; PACE, carbohydrate a
The major mRNP protein YB-1: Structural and association properties in solution
Keywords: FPLC، کروماتوگرافی مایع سریع پروتئین; UV, ultraviolet; CD, circular dichroism; DSC, differential scanning calorimetry; NMR, nuclear magnetic resonance; SAXS, small-angle X-ray scattering; FPLC, fast protein liquid chromatography; CSD, cold shock domain; GuHCl, guanidine hydrochloride; BSA, bo
Characterization of Oncorhynchus mykiss 5-hydroxyisourate hydrolase/transthyretin superfamily: Evolutionary and functional analyses
Keywords: FPLC، کروماتوگرافی مایع سریع پروتئین; A, absorbance; aa, amino acid; ANOVA, analysis of variance; BSA, bovine serum albumin; cDNA, DNA complementary to RNA; EST, expressed sequence tag; FPLC, fast protein liquid chromatography; 5-HIU, 5-hydroxyisourate; HIUHase, 5-hydroxyisourate hydrolase; H
Mouse serum paraoxonase-1 lactonase activity is specific for medium-chain length fatty acid lactones
Keywords: FPLC، کروماتوگرافی مایع سریع پروتئین; DTPA, diethylenetriamine pentaacetic acid; EDTA, ethylenediaminetetraacetic acid; FPLC, Fast Protein Liquid Chromatography; HETE, hydroxyeicosatetraenoate; HETEL, hydroxyeicosatetraenoate delta lactone; HDL, high-density lipoprotein; MOPS, 3-(N-morpholino
Chemical characterization of pro-inflammatory amyloid-beta peptides in human atherosclerotic lesions and platelets
Keywords: FPLC، کروماتوگرافی مایع سریع پروتئین; Aβ, amyloid-beta; AD, Alzheimer's disease; ApoE, apolipoprotein E; APP, amyloid precursor protein; AVD, atherosclerotic vascular disease; BBB, blood–brain barrier; CAA, cerebral amyloid angiopathy; FPLC, fast protein liquid chromatography; GDFA, glass-dis
Cysteinemia, rather than homocysteinemia, is associated with plasma apolipoprotein A-I levels in hyperhomocysteinemia: Lipid metabolism in cystathionine β-synthase deficiency
Keywords: FPLC، کروماتوگرافی مایع سریع پروتئین; Apo, apolipoprotein; CBS, cystathionine β-synthase; FPLC, fast protein liquid chromatography; GSH, Glutathione; Hcy, homocysteine; HDL, high density lipoproteins; IL-6, interleukin 6; MCP-1, monocyte chemotactic protein-1; PCR, polymerase chain reaction;
Long bone osteoclasts display an augmented osteoclast phenotype compared to calvarial osteoclasts
Keywords: FPLC، کروماتوگرافی مایع سریع پروتئین; TRAP, tartrate-resistant acid phosphatase; FPLC, fast protein liquid chromatography; AMC, 7-amino-4-methylcoumarin; CtsK, cathepsin KTartrate-resistant acid phosphatase; Purple acid phosphatase; Acp 5; Cysteine proteinase; Osteoclast
Digestive amylase of a primitive animal, the scorpion: Purification and biochemical characterization
Keywords: FPLC، کروماتوگرافی مایع سریع پروتئین; ELISA, enzyme linked immunosorbent assay; FPLC, fast protein liquid chromatography; SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis; SDA, scorpion digestive amylase; BLA, Bacillus liqueniformis amylase; BSA, bovine serum albumine; PPA,
Structural and mechanistic insights into a novel non-competitive Kunitz trypsin inhibitor from Adenanthera pavonina L. seeds with double activity toward serine- and cysteine-proteinases
Keywords: FPLC، کروماتوگرافی مایع سریع پروتئین; ApTKI, Adenanthera pavonina trypsin Kunitz inhibitor; FPLC, fast protein liquid chromatography; PjTKI, Prosopis juliflora trypsin Kunitz inhibitor; SKTI, soybean Kunitz trypsin inhibitor; TCA, trichloroacetic acidAdenanthera pavonina; Non-competitive prot
Biogenesis of tartrate-resistant acid phosphatase isoforms 5a and 5b in stably transfected MDA-MB-231 breast cancer epithelial cells
Keywords: FPLC، کروماتوگرافی مایع سریع پروتئین; TRAP, tartrate-resistant acid phosphatase; FPLC, fast protein liquid chromatography; BfA, Brefeldin A; Con A, Concanavalin A; pNPP, p-nitrophenylphosphate; PVDF, polyvinylidene fluoride; AMC, 7-amino-4-methylcoumarinTartrate-resistant acid phosphatase; Pu
Modulation of interleukin signalling and gene expression in cardiac myocytes by endothelin-1
Keywords: FPLC، کروماتوگرافی مایع سریع پروتئین; ERK, extracellular signal-regulated kinase; ET-1, endothelin-1; FPLC, fast protein liquid chromatography; GqPCR, Gq protein-coupled receptor; IL, interleukin; IL-1R, IL-1 receptor; IL-1RAcP, IL-1R accessory protein; JNK, c-Jun N-terminal kinase; MAPK, mit
Review of preparative and analytical procedures for the study of proteins in grape juice and wine
Keywords: FPLC، کروماتوگرافی مایع سریع پروتئین; PR, pathogenesis-related; SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis; RP-HPLC, reversed phase high performance liquid chromatography; ELISA, enzyme-linked immunosorbent assay; FPLC, Fast Protein Liquid Chromatography; HPSEC, high
Characterization of the expression and regulation of MK5 in the murine ventricular myocardium
Keywords: FPLC، کروماتوگرافی مایع سریع پروتئین; DMSO, dimethylsulfoxide; DTT, dithiothreitol; ERK, extracellular signal-regulated kinase; FPLC, fast protein liquid chromatography; GST, glutathione S-transferase; MAP kinase, mitogen-activated protein kinase; MK2, MAP kinase-activated protein kinase-2; M
Formation of preβ1-HDL during lipolysis of triglyceride-rich lipoprotein
Keywords: FPLC، کروماتوگرافی مایع سریع پروتئین; HDL, high-density lipoprotein; CHO, cholesterol; TG, triglyceride; NEFA, nonesterified fatty acids; FPLC, fast protein liquid chromatography; LPL, lipoprotein lipase; LDL, low-density lipoprotein; VLDL, very low-density lipoprotein; apoA-I, apolipoprotein
Effect of soya protein on the dialysability of exogenous iron and zinc
Keywords: FPLC، کروماتوگرافی مایع سریع پروتئین; FeSO4, ferrous sulphate; ZnO, zinc oxide; SDS–PAGE, sodium dodecyl sulphate–polyacrylamide gel electrophoresis; FPLC, fast protein liquid chromatography; kDa, kiloDaltons; AAS, atomic absorption spectroscopySoya flour; Soya concentrate; Soya isolate; Iron
A Kazal-type serine proteinase inhibitor from chicken liver (clTI-1): Purification, primary structure, and inhibitory properties
Keywords: FPLC، کروماتوگرافی مایع سریع پروتئین; clTI-1, chicken liver trypsin inhibitor; PSTI, pancreatic secretory trypsin inhibitor; HPLC, high-performance liquid chromatography; FPLC, fast protein liquid chromatography; BApNA, N-α-benzoyl-dl-arginine-p-nitroanilide; NPGB, p-nitrophenyl p-guanidinobe
A novel oxygenase from Pleurotus sapidus transforms valencene to nootkatone
Keywords: FPLC، کروماتوگرافی مایع سریع پروتئین; aa, amino acids; CIS, cooled injection system; CV, column volume; DEAE, diethylaminoethyl; FPLC, fast protein liquid chromatography; IEX, ion exchange chromatography; MWCO, molecular weight cut off; TMBZ, tetramethylbenzidineBasidiomycete; Biotransformati
Purification, characterization and cloning of a ricin B-like lectin from mushroom Clitocybe nebularis with antiproliferative activity against human leukemic T cells
Keywords: FPLC، کروماتوگرافی مایع سریع پروتئین; CNL, Clitocybe nebularis lectin; RP-HPLC, reversed-phase high-performance liquid chromatography; TFA, trifluoroacetic acid; SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis; FPLC, fast protein liquid chromatography; PCR, polymerase chai
Sulfated galactan is a catalyst of antithrombin-mediated inactivation of α-thrombin
Keywords: FPLC، کروماتوگرافی مایع سریع پروتئین; APTT, activated partial thrombosplastin time; FPLC, fast protein liquid chromatography; SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis; HPLC, high pressure liquid chromatographyAnticoagulant activity; Algal polysaccharide; Exosite II
The origin and early development of wheat glutenin particles
Keywords: FPLC، کروماتوگرافی مایع سریع پروتئین; GMP; Protein bodies; Wheat; OriginCSLM, confocal laser scanning microscopy; DAF, days after flowering; ER, endoplasmic reticulum; FITC, fluorescent protein label; FPLC, fast protein liquid chromatography; GMP, glutenin macropolymer; HMW, high molecular we
Characterization of monoclonal antibodies to equine interleukin-10 and detection of T regulatory 1 cells in horses
Keywords: FPLC، کروماتوگرافی مایع سریع پروتئین; DC, dendritic cell; FPLC, fast protein liquid chromatography; IGHG4, immunoglobulin heavy chain gamma 4 constant gene; TR1 cells, T regulatory 1 cells; Treg cells, regulatory T-cellsInterleukin-10; Equine cytokines; Flow cytometry; ELISA; TR1 cells
The activities of nucleoside diphosphate kinase and adenylate kinase are influenced by their interaction
Keywords: FPLC، کروماتوگرافی مایع سریع پروتئین; AK, adenylate kinase; Ap5a, P5-di(adenosine 5′)-pentaphosphate; FPLC, fast protein liquid chromatography; IMS, inter membrane space; LDH, lactate dehydrogenase; MPSS, massively parallel signature sequencing; NDPK, nucleoside diphosphate kinase; NHS, N-hyd
Quinol type compound in cytochrome c preparations leads to non-enzymatic reduction of cytochrome c during the measurement of complex III activity
Keywords: FPLC، کروماتوگرافی مایع سریع پروتئین; FPLC, fast protein liquid chromatography; HB-cyt c, high-background cytochrome c; IFM, interfibrillar mitochondria; LB-cyt c, low-background cytochrome c; MS, mass spectrometry; SDS–PAGE, sodium dodecylsulfate–polyacrylamide gel electrophoresis; SOD, supe
VHH, bivalent domains and chimeric Heavy chain-only antibodies with high neutralizing efficacy for scorpion toxin AahI′
Keywords: FPLC، کروماتوگرافی مایع سریع پروتئین; Aah, Androctonus autralis hector scorpion; AahG50, Sephadex G-50 gel filtration toxic fraction of Aah venom; ELISA, enzyme-linked immunoabsorbent assay; FPLC, Fast Protein Liquid Chromatography; HCAb, Heavy-chain antibody; HPLC, High Performance Liquid Ch
Contributions of Zn(II)-binding to the structural stability of endostatin
Keywords: FPLC، کروماتوگرافی مایع سریع پروتئین; ES, endostatin; FZ, FluoZin-1, tripotassium salt; CD, circular dichroism; FPLC, fast protein liquid chromatography; GdmC1, guanidine hydrochloride; DSC, differential scanning calorimetry; ZBP, Zn(II)-binding peptide; ZBP-ES, Zn(II)-binding peptide modifie
Identification and stability of trypsin inhibitor isoforms in pea (Pisum sativum L.) cultivars grown in New Zealand
Keywords: FPLC، کروماتوگرافی مایع سریع پروتئین; TIA, trypsin inhibitor activity; TIU/mg DM, trypsin inhibitor units per milligram of dry matter; PPI, pea protease inhibitor; BBI, Bowman-Birk inhibitor; FPLC, fast protein liquid chromatography; IEF, isoelectric focusing; SDS–PAGE, SDS–polyacrylamide gel
Purification and partial characterization of two phospholipases A2 from Bothrops leucurus (white-tailed-jararaca) snake venom
Keywords: FPLC، کروماتوگرافی مایع سریع پروتئین; Bothrops leucurus; PLA2; Platelet aggregation; Blood coagulation; Angiogenesis; Snake venomAA, arachidonic acid; ADP, adenosine 5′-diphosphate; ELISA, enzyme-linked immunosorbent assay; FPLC, fast protein liquid chromatography; FUNED, Fundacao Ezequiel Di
Bowman–Birk protease inhibitor from the seeds of Vigna unguiculata forms a highly stable dimeric structure
Keywords: FPLC، کروماتوگرافی مایع سریع پروتئین; BBI-Bowman, Birk protease inhibitor; PIs, Protein Protease inhibitors; ES-MS, Electrospray ionization mass spectrometry; SDS-PAGE, Sodium dodecyl sulfate-polyacrylamide gel electrophoresis; FPLC, Fast protein liquid chromatography; BT, Bovine Trypsin; NCS
Protein chemical characterization of Gc globulin (vitamin D-binding protein) isoforms; Gc-1f, Gc-1s and Gc-2
Keywords: FPLC، کروماتوگرافی مایع سریع پروتئین; BCIP/NBT, 5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium; CIE, crossed immunoelectrophoresis; DBP, vitamin D-binding protein; DTT, dithiothreitol; ESI, electro spray ionization; FPLC, fast protein liquid chromatography; Gc, group-specific com
Antibody internalization studied using a novel IgG binding toxin fusion
Keywords: FPLC، کروماتوگرافی مایع سریع پروتئین; CDR, complementarity determining region; DMF, Dimethyl Formamide; ELISA, enzyme-linked immunosorbent assay; FCS, fetal calf serum; FITC, fluorescein isothiocyanate; mAb, monoclonal antibody; FPLC, fast protein liquid chromatography; HRP, horseradish perox
Mesangial autoantigens in IgA nephropathy: matrix synthesis and localization
Keywords: FPLC، کروماتوگرافی مایع سریع پروتئین; ELISA, enzyme-linked immunosorbent assay; FCS, foetal calf serum; FPLC, fast protein liquid chromatography; GAG, glycosaminoglycan; Igγ, interferon gamma; IgA-N, IgA nephropathy; IgG-MESCA, IgG anti-mesangial cell antibodies; LPS, lipopolysaccharide; OD,
Purification and kinetic properties of elisabethatriene synthase from the coral Pseudopterogorgia elisabethae
Keywords: FPLC، کروماتوگرافی مایع سریع پروتئین; CHAPS, 3-[(3-Cholamidopropyl)dimethylammonio]-1-propane-sulfonate; Dpm, Disintegrations/minute; DTT, Dithiothreitol; ELS, Elisabethatriene synthase; FPLC, Fast protein liquid chromatography; Km, Michaelis constant; K-P, Phosphate buffer; kcat, Turnover nu
Reconstitution of Calmodulin from Domains and Subdomains: Influence of Target Peptide
Keywords: FPLC، کروماتوگرافی مایع سریع پروتئین; protein reconstitution; Ca2+-binding protein; EF-hand; calmodulin; synthetic peptidesCaM, calmodulin (Ca2+-saturated form); ITC, isothermal titration calorimetry; FPLC, fast protein liquid chromatography; HSQC, heteronuclear single-quantum coherence; smML