Development of an infectious clone and replicon system of norovirus GII.4
Keywords: Norwalk virus; Caliciviridae; Full-length cDNA clone; Seamless cloning;
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Construction and characterization of HIV type 1 CRF07_BC infectious molecular clone from men who have sex with men
Keywords: HIV-1; CRF07_BC; Men who have sex with men; Infectious molecular clone; Full-length cDNA clone; Long terminal repeat;
Construction of an infectious clone of a plant RNA virus in a binary vector using one-step Gibson Assembly
Keywords: Gibson Assembly; Seamless cloning; Full-length cDNA clone; Tymovirus;
Construction of infectious cDNA clone derived from a classical swine fever virus field isolate in BAC vector using in vitro overlap extension PCR and recombination
Keywords: BAC; bacterial artificial chromosome; CSFV; classical swine fever virus; FAT; fluorescent antibody test; UTRs; un-translated regions; ORF; open reading frame; PK15; Porcine kidney cells; RT-PCR; reverse transcriptase PCR; vRNA; viral RNA; Classical swine
A simplified approach to construct infectious cDNA clones of a tobamovirus in a binary vector
Keywords: Tobamovirus; PMMoV; Full-length cDNA clone; Overlap-extension PCR;
Rescue of the highly virulent classical swine fever virus strain “Koslov” from cloned cDNA and first insights into genome variations relevant for virulence
Keywords: Flaviviridae; Pestivirus; Classical swine fever virus; CSFV; Koslov; Full-length cDNA clone; Virulence; Pathogenesis; Viral populations; Next generation sequencing;
Construction and properties of a gene-silencing vector based on Poplar mosaic virus (genus Carlavirus)
Keywords: Virus-induced gene-silencing; Poplar mosaic carlavirus; High-fidelity long PCR; Full-length cDNA clone;